Our experienced staff of molecular biologists have worked to provide you with a set of optimized protocols for the homogenization of various tissue, cell types, and organisms so you can spend less time troubleshooting and more time getting results. Most protocols have been independently verified.

All of our currently available protocols are listed below. Please read the General Guidelines before using the Bullet Blender™ in any application.

Bullet Blender™ 5 protocols: Use the protocols for the standard Bullet Blender™ and scale up as appropriate.

General Guidelines - Please Read First!
Types of Samples	Bullet Blender™ (1.5 to 2.0 mL microcentrifuge tubes)	Bullet Blender™ 50 (50 mL centrifuge tubes)
In General...	Cell culture, soft or hard tissue, whole organisms	Cell culture, soft tissue, organisms without a cuticle
Cell Culture	Candida albicans Drosophila S2 cells E. Coli Mammalian Cell Culture Saccharomyces cerevisiae	E. Coli Saccharomyces cerevisiae
Animal Tissue	Brain Cancer Tissue (Carcinoma) Cartilage Epithelium Fat / Adipose Hair Heart Kidney Liver Lung/Trachea Lymph Node Meconium Muscle (Striated) Pharynx Tail Snips Skin Spleen Stomach / Jejunum Uterus	Fat / Adipose
Plant Tissue	A. Thaliana Blueberry (Vaccinium) Ginger Rhizome (Zingiber officinale) Leek Leaves (Allium ampeloprasum) Horseradish Root (Armoracia rusticana) Malanga Tuber (Xanthosoma sagittifolium) Pine Nut (Pinus) Soy Bean (G. Max) Sugar Cane Pulp (Saccharum)	Contact us for recommendations
Small Organisms	Honeybee Apis mellifera Roundworm C. Elegans Zebrafish Danio Rerio Fruit Fly (adult) Drosophila Fruit Fly (larvae) Drosophila	Shellfish
Extraction of small cells	Lymphocyte Extraction from Heart Splenocyte Extraction from Spleen	Contact us for recommendations
Protocols for Use with Kits	QuickGene Mini80 - Tissue QuickGene Mini80 - Cultured Cells	Contact us for recommendations
Need more Help? Please check out our handy Troubleshooting and Maintenance Guide.
Adobe Acrobat Reader required to view protocols. Click Here to download Adobe PDF Reader

If you need help developing an assay with the Bullet Blender™, feel free to e-mail our tech support at support@NextAdvance.com or fill out our support form and we'll do our best to assist you.

If you have developed a protocol with the bullet blender that is not listed on this page, or you have developed a protocol that works better for certain applications, please share it with everyone! E-mail it to us at support@NextAdvance.com or send it using our support form and we'll review it and add it to our list of protocols. Don't worry - we'll acknowledge you for it! Thanks!

PLEASE NOTE: The protocols used with the Bullet Blender are different than protocols used with other bead-mill homogenizers. If you previously used another product, please be sure not to use its protocols with the Bullet Blender, as you will very likely experience different results.

 

Does the protocol use a different kind of bead than the type you currently have? Click here for pricing and ordering.