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>
No Cross Contamination
The samples are homogenized (or mixed)
non-invasively. The sample tubes are kept closed during
agitation, as the samples are processed. There are no probes to
clean between samples.
> Samples Stay Cool
The instrument uses very little power due to the
unique, patented design. It uses a small DC motor to agitate the
individual tubes, not ultrasonics. Also, because the motor
does not need to agitate any heavy platforms or plates, it is small and
will last for years.
>
Convenient
to Use
Simply place your lymph nodes and other tissue samples or
cell cultures and some beads in standard high quality polypropylene
tubes, and load the tubes into the Bullet Blender™.
Set the duration (typically a few minutes) and speed
(vigorousness). There are no probes to clean. And it is
quieter than a sonicator and does not heat up your samples more than a
few degrees!
>
Risk Free
Purchase
The Bullet
Blender™ comes with a 30-day
money back guarantee and a two year warranty, with a three year
warranty on the motor. The simple, reliable design enables the Bullet Blenders™ to sell for a fraction
of the price of ultrasonic or other agitation based instruments, yet
provides an easier, quicker technique.
> Established Protocols
Protocol for Lymphatic Tissue Homogenization in the Bullet Blender™
The protocol described in this document is for the use of the Bullet Blender™ for the
homogenization of lymph nodes (from a variety of animals). Note that the time and
speed settings may differ due to the variation in consistency / texture of tissue from
species to species. This protocol does not specify a particular buffer - you may choose
which is most appropriate for your downstream application (nucleic acid isolation, protein
extraction, etc.).
Materials Required:
lymph node tissue, microcentrifuge tubes, Bullet Blender™,
homogenization buffer, pipettor, and 0.5mm zirconium oxide
beads (part number ZrOB05)
Instructions:
- Cut lymph tissue into appropriately sized pieces for analysis (50mg-300mg) and
place into a microcentrifuge tube. Lymph nodes vary widely in size (from 5mg to
150mg).
- OPTIONAL: Wash tissue with ~1mL PBS. Aspirate. NOTE: This step removes
external contaminants (blood, etc.).
- Add zirconium oxide beads (0.5mm). Use a mass of beads equal to 2x your mass of
tissue. One scoop of beads ≈ 190mg.
- Add 0.1mL to 0.6mL buffer (2 volumes of buffer for every mass of tissue).
- Close the microcentrifuge tubes.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 8 and TIME 3 minutes. Press Start.
- After the run, remove tubes from the instrument.
- Visually inspect samples. If homogenization is unsatisfactory, run for another two
minutes at the SPEED 10.
- Proceed with your downstream application.
SAFETY NOTE!!!
When using a centrifuge to separate your homogenate from
the debris and beads, make sure your tubes are balanced.
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