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>
No Cross Contamination
The samples are homogenized (or mixed)
non-invasively. The sample tubes are kept closed during
agitation, as the samples are processed. There are no probes to
clean between samples.
> Samples Stay Cool
The instrument uses very little power due to the
unique, patented design. It uses a small DC motor to agitate the
individual tubes, not ultrasonics. Also, because the motor
does not need to agitate any heavy platforms or plates, it is small and
will last for years.
>
Convenient
to Use
Simply place your beans and other tissue samples or
cell cultures and some beads in standard high quality polypropylene
tubes, and load the tubes into the Bullet Blender™.
Set the duration (typically a few minutes) and speed
(vigorousness). There are no probes to clean. And it is
quieter than a sonicator and does not heat up your samples more than a
few degrees!
>
Risk Free
Purchase
The Bullet
Blender™ comes with a 30-day
money back guarantee and a two year warranty, with a three year
warranty on the motor. The simple, reliable design enables the Bullet Blenders™ to sell for a fraction
of the price of ultrasonic or other agitation based instruments, yet
provides an easier, quicker technique.
> Established Protocols
Protocol for Soybean Homogenization in the Bullet Blender™
The protocol described in this document is for the use of the Bullet Blender™ for the
homogenization of soybean (seeds from Glycine max). Note that the time and speed
settings may differ due to the variation in consistency/texture of different subspecies.
This protocol does not specify a particular buffer - you may choose which is most
appropriate for your downstream application (nucleic acid isolation, protein extraction,
etc.).
Materials Required:
soybean, saline, Bullet Blender™, homogenization buffer, pipetor,
microcentrifuge tubes, 0.5mm zirconium oxide beads (part
number ZrOB05)
Instructions:
- Place one soybean (100-200mg) into a microcentrifuge tube.
- OPTIONAL: Wash 3x with ~1mL PBS. Aspirate. NOTE: This step removes some
external contaminants and debris. Additional washes may be performed as desired.
- Add a 0.6g of zirconium oxide beads (0.5mm) to the tube. One scoop of beads ≈ 190mg.
- Add 0.4mL buffer to the tube.
- Close the microcentrifuge tubes.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 10 and TIME 4 minutes. Press Start.
- After the run, remove tubes from the instrument.
- Visual inspection of the homogenate is difficult due to the formation of emulsions
with the oils and the high protein content from the plant tissue in aqueous buffers.
To verify sufficient homogenization, you may place forceps or a spatula inside the
tube to search for remaining large fragments. If homogenization is unsatisfactory,
run for another two minutes at the SPEED 10.
- Proceed with your downstream application.
SAFETY NOTE!!!
When using a centrifuge to separate your homogenate from
the debris and beads, make sure your tubes are balanced.
Typical Results
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