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>
No Cross Contamination
The samples are homogenized (or mixed)
non-invasively. The sample tubes are kept closed during
agitation, as the samples are processed. There are no probes to
clean between samples.
> Samples Stay Cool
The instrument uses very little power due to the
unique, patented design. It uses a small DC motor to agitate the
individual tubes, not ultrasonics. Also, because the motor
does not need to agitate any heavy platforms or plates, it is small and
will last for years.
>
Convenient
to Use
Simply place your small organisms, tissue samples, or
cell cultures along with some beads into standard high quality polypropylene
tubes, and load the tubes into the Bullet Blender™.
Set the duration (typically a few minutes) and speed
(vigorousness). There are no probes to clean, it is
quieter than a sonicator and does not heat up your samples more than a
few degrees!
>
Risk Free
Purchase
The Bullet
Blender™ comes with a 30-day
money back guarantee and a two year warranty, with a three year
warranty on the motor. The simple, reliable design enables the Bullet Blenders™ to sell for a fraction
of the price of ultrasonic or other agitation based instruments, yet
provides an easier, quicker technique.
> Established Protocols
Researchers are already getting publication-quality results from the homogenization of Honeybee samples with the Bullet Blender!
The Bullet
Blender™ home page shows the entire family of products and links to
other applications.
Protocol for Apis mellifera Homogenization in the Bullet Blender
The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of Apis mellifera thoraces. This protocol was created for the extraction of DNA, and does specify a particular buffer, however you may modify it in any way necessary to tailor it to your needs (RNA extraction, protein purification, etc.).
Materials Required:
Apis mellifera, Bullet Blender™, homogenization buffer, pipettor, microcentrifuge tubes, and 1.0mm glass beads (part number GB10).
Instructions:
- Wash A. mellifera in PBS or other buffer, as appropriate, to remove food, surface bacteria, and other contaminants.
- Isolate the thorax using a scalpel and forceps.
- Place each thorax, individually, into a microcentrifuge tube.
- Add 100mg of 1.0mm glass beads to the tube. One scoop ≈ 68mg.
- Add 600ml of lysis buffer (100 mM Tris, pH 8.0,10 mM EDTA, pH 8.0, and 1% SDS).
- Close the microcentrifuge tubes.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 8 and TIME 3 minutes. Press Start.
- After the run, remove the tubes from the instrument.
- Visually inspect samples. If homogenization is unsatisfactory, run for another two minutes at the SPEED 10.
- Proceed with your downstream application.
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