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Innovative Lab Products for the Life Sciences

The Bullet Blender™ and Bullet Blender™ Blue
The easiest and coolest way to harvest DNA, RNA and proteins from fruit

No chemical lysis or harsh detergents required!
Your fruit samples stay cool!


The Bullet Blender™Lyse E. coli and other cells. Homogenize or disrupt tissue. The Bullet Blender is quiet and user friendly.

> No Cross Contamination
> Samples stay cool
> Convenient
> Risk  Free
> Established protocols

Homogenize up to 24 blueberry samples simultaneously-- in less than 3 minutes!

 

The Bullet Blender™ enables you to homogenize up to 24 samples (blueberry or other fruit) in separate microcentrifuge tubes at a time.  Load up to 300mg blueberry (including skin and seeds) with your application buffer and grinding beads in standard polypropylene microcentrifuge tubes.  Place the tubes into the Bullet Blender™ (pictured above) or Bullet Blender™ Blue.  The "bullets" in the "blender" vigorously strike all the sample tubes as the instrument runs for a few minutes.  The agitation of the grinding agents with the tissues homogenizes the samples.

The Bullet Blender™ and Bullet Blender™ Blue enable you to disrupt or homogenize up to 24 berry and other tissue/cell samples at a time.  Load the samples in standard polypropylene microcentrifuge tubes into the Bullet Blender ™.  Balls (the "bullets") repeatedly strike the sample tubes in a controlled manner, thousands of times per minutes, inducing vigorous motion of the kidney samples or other substances inside the tubes, providing efficient mixing. With beads in the tubes, the samples are thoroughly homogenized. Tune the extent of disruption by adjusting the speed. Click here to see sample protocols.

> No Cross Contamination

The samples are homogenized (or mixed) non-invasively.  The sample tubes are kept closed during agitation, as the samples are processed.  There are no probes to clean between samples.

> Samples Stay Cool

The instrument uses very little power due to the unique, patented design.  It uses a small DC motor to agitate the individual tubes, not ultrasonics.   Also, because the motor does not need to agitate any heavy platforms or plates, it is small and will last for years.

> Convenient to Use

Simply place your berry and other fruit samples or cell cultures and some beads in standard high quality polypropylene tubes, and load the tubes into the Bullet Blender™.   Set the duration (typically a few minutes) and speed (vigorousness).  There are no probes to clean.  And it is quieter than a sonicator and does not heat up your samples more than a few degrees!

> Risk Free Purchase

The Bullet Blender™ comes with a 30-day money back guarantee and a two year warranty, with a three year warranty on the motor.  The simple, reliable design enables the Bullet Blender™ to sell for a fraction  of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.

> Established Protocols

Blueberry:  Place about 100mg of fruit in each microcentrifuge tube. Load 100-150mg stainless steel bead blend (0.9-2.0mm dia) in each tube.  Add 100-200μL buffer (your choice, depending on your downstream application), then place the tubes into the Bullet Blender™.  Set the time to 3 minutes and the speed to 10.

 

 

The Bullet Blender™ home page shows the entire family of products and links to other applications.

Protocol for Blueberry Homogenization in the Bullet Blender

The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of blueberry (flesh, seeds and skin form the genus Vaccinium L.). This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.).

Materials Required:

blueberry, saline, aspirator, Bullet Blender™, homogenization buffer, pipettor, microcentrifuge tubes, and 0.9-2.0mm stainless steel bead blend (part number SSB14B).

Instructions:
  1. OPTIONAL: Wash blueberry 3x with ~1mL PBS. Aspirate. NOTE: This step removes external contaminants and debris.
  2. Section blueberry into quarters. Place quarter (100-200mg) into a microcentrifuge tube. Size may vary depending on species.
  3. Add a mass of stainless steel bead blend equal to 1.5X the mass of fruit. One scoop of beads ≈ 200mg.
  4. Add 0.2mL to 0.6mL buffer, i.e. 2 volumes of buffer for every mass of fruit.
  5. Close the microcentrifuge tubes.
  6. Place tubes into the Bullet Blender™.
  7. Set controls for SPEED 8 and TIME 3 minutes. Press Start.
  8. After the run, remove the tubes from the instrument.
  9. Visually inspect samples. If homogenization is unsatisfactory, run for another three minutes at the SPEED 10.
  10. Proceed with your downstream application.

SAFETY NOTE!!!

When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced.