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No Cross Contamination
The samples are homogenized (or mixed) non-invasively. The
sample tubes are kept closed during agitation, as the
samples are processed. There are no probes to
clean between samples.
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Samples Stay
Cool
The instrument uses very
little power due to the unique, patented design.
It uses a small DC motor to agitate the individual
tubes, not ultrasonics. Also, because
the motor does not need to agitate any heavy
platforms or plates, it is small and will last for
years.
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Convenient to Use
Simply place your kidney and other tissue samples or cell cultures and some beads in standard
high quality polypropylene tubes, and load the tubes into the
Bullet Blender™. Set the duration (typically a few
minutes) and speed (vigorousness). There are no probes to clean.
And it is quieter than a sonicator and does not heat up
your samples more than a few degrees!
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Risk Free Purchase
The Bullet Blender™
comes with a 30-day money back guarantee and a two year
warranty, with a three year warranty on the motor. The simple, reliable design enables the
Bullet Blenders™ to
sell for a fraction of the price of ultrasonic or
other agitation based instruments, yet provides an easier,
quicker technique.
> Established Protocols
Brain:
Place about 100mg of dissected tissue
into each microcentrifuge
tube. Load 150mg zirconium oxide beads (0.5 mm dia). Add 200-600μL buffer (your choice, depending on your
downstream application), then place the tube into the Bullet Blender™.
Set the time to 4 minutes and the speed to 8.
The Bullet Blender™ home page shows the entire family of products and links to other applications.
Protocol for Kidney Tissue Homogenization in the Bullet Blender™
The protocol described in this document is for the use of the Bullet Blender for the
homogenization of kidney tissue from a variety of animals. Note that the time and
speed settings may differ due to the variation in consistency/texture of tissue from
species to species. This protocol does not specify a particular buffer - you may choose
which is most appropriate for your downstream application (nucleic acid isolation,
protein extraction, etc.).
Materials Required:
kidney tissue, saline, aspirator, Bullet Blender™, homogenization buffer, pipettor,
microcentrifuge tubes, and 0.5 mm zirconium oxide beads (part number
ZROB05).
Instructions:
- Cut brain tissue into appropriately sized pieces for
analysis (50mg-300mg) and place into a microcentrifuge tube.
- OPTIONAL: Wash tissue 3x with ~1mL PBS. Aspirate. NOTE: This step
removes external contaminants (blood, etc.).
- Add a mass of the zirconium beads (0.5mm) equal to 1.5X the mass of tissue. One scoop of beads ≈ 190mg.
- Add 0.2mL to 0.6mL buffer, i.e. 2 volumes of buffer for every mass of sample.
- Close the microcentrifuge tubes.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 8 and TIME4 minutes. Press Start.
- After the run, remove the tubes from the instrument.
- Visually inspect samples. If homogenization is unsatisfactory, run for another three minutes at the SPEED 10.
- Proceed with your downstream application.
SAFETY NOTE!!!
When using a centrifuge to separate your homogenate from
the debris and beads, make sure your tubes are balanced.
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