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Innovative Lab Products for the Life Sciences

The Bullet Blender™ and Bullet Blender™ Blue

Would you like crackers with your pâté?

Your LIVER samples stay cool
during homogenization!


The Bullet Blender™Lyse E. coli and other cells. Homogenize or disrupt tissue. The Bullet Blender is quiet and user friendly.

> No Cross Contamination
> Samples stay cool
> Convenient
> Risk  Free
> Established protocols

Homogenize up to 24 liver samples simultaneously-- in less than 3 minutes!

 

The Bullet Blender™ enables you to homogenize up to 24 samples (liver or other tissues) in separate microcentrifuge tubes at a time.  Load up to 300mg liver tissue with your application buffer and grinding beads in standard polypropylene microcentrifuge tubes.  Place the tubes into the Bullet Blender™ (pictured above) or Bullet Blender™ Blue.  The "bullets" in the "blender" vigorously strike all the sample tubes as the instrument runs for a few minutes.  The agitation of the grinding agents with the tissues homogenizes the samples.

The Bullet Blender™ and Bullet Blender™ Blue enable you to disrupt or homogenize up to 24 liver and other tissue/cell samples at a time.  Load the samples in standard polypropylene microcentrifuge tubes into the Bullet Blender™.  Balls (the "bullets") repeatedly strike the sample tubes in a controlled manner, thousands of times per minutes, inducing vigorous motion of the tissue samples or other substances inside the tubes, providing efficient mixing. With beads in the tubes, the samples are thoroughly homogenized. Tune the extent of disruption by adjusting the speed. Click here to see sample protocols.

> No Cross Contamination

The samples are homogenized (or mixed) non-invasively.  The sample tubes are kept closed during agitation, as the samples are processed.  There are no probes to clean between samples.

> Samples Stay Cool

The instrument uses very little power due to the unique, patented design.  It uses a small DC motor to agitate the individual tubes, not ultrasonics.   Also, because the motor does not need to agitate any heavy platforms or plates, it is small and will last for years.

> Convenient to Use

Simply place your liver and other tissue samples or cell cultures and some beads in standard high quality polypropylene tubes, and load the tubes into the Bullet Blender™.   Set the duration (typically a few minutes) and speed (vigorousness).  There are no probes to clean.  And it is quieter than a sonicator and does not heat up your samples more than a few degrees!

> Risk Free Purchase

The Bullet Blender™ comes with a 30-day money back guarantee and a two year warranty, with a three year warranty on the motor.  The simple, reliable design enables the Bullet Blenders™ to sell for a fraction  of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.

> Established Protocols

Liver : 200 mg of beef liver cut into several pieces with equal volume of 0.5mm zirconium silicate or zirconium oxide beads and 0.4 mL buffer.  2 minutes at speed 8.

Protein quantification results of lysates from an experiment using  0.3g of 0.5mm glass beads.  (Zirconium silicate and zirconium oxide beads work even better.):


 Time
(min)		Sample 1
(mg/mL)		Sample 2
(mg/mL)		Notes (glass bead extraction)

02.621.00protein present from periphery of tissue

 16.808.11 

 29.4210.46 

 310.5410.11buffer saturated with protein

liver before homogenization liver ZrO after homogenization
Liver (0.3g) before homogenization
w/ZrO beads (1mm, 0.3g)
w/0.6mL buffer (0.5% NP-40)		 Protein
concentration
(mg/mL)
 27.6
 28.4
 31.0
 23.3
After liver homogenization
w/ZrO beads, 3 minutes speed 8,
centrifuged 15 minutes @ 14K rpm

The Bullet Blender™ home page shows the entire family of products and links to other applications.



Protocol for Hepatic (Liver) Tissue Homogenization in the Bullet Blender™

The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of liver / hepatic tissue (from a variety of animals). Note that the time and speed settings may differ due to the variation in consistency / texture of liver tissue from species to species. This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.).

Materials Required:

liver tissue, saline, Bullet Blender™, homogenization buffer, pipettor, microcentrifuge tubes, zirconium oxide beads (0.5mm or 1.0mm) or zirconium silicate beads (0.5 or 1.0mm).

Instructions:
  1. Cut liver into appropriately sized pieces for analysis (50mg-300mg) and place into a microcentrifuge tube.
  2. OPTIONAL: Wash tissue 3x with ~1mL PBS. Aspirate. NOTE: This step removes external contaminants (blood, etc.).
  3. Add zirconium oxide beads (0.5mm or 1.0mm) OR zirconium silicate beads (0.5mm or 1.0mm) to the tube. Use a mass of beads equal to 1.5x your mass of tissue.
  4. Add 0.1mL to 0.6mL buffer (2 volumes of buffer for every volume of cells).
  5. Close the microcentrifuge tubes.
  6. Place tubes into the Bullet Blender™.
  7. Set controls for SPEED 8 and TIME 3 minutes. Press Start.
  8. After the run, remove tubes from the instrument.
  9. Visually inspect samples. If homogenization is unsatisfactory, run for another two minutes at the SPEED 8.
  10. Proceed with your downstream application.
SAFETY NOTE!!!

When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced.

Typical Results
Typical Results



Bullet Blender™ 50 Homogenization Protocol for Liver

The protocol described in this document is for the use of the Bullet Blender™ 50 for the homogenization of liver / hepatic tissue. This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.).

Materials Required:

liver , Bullet Blender™ 50, homogenization buffer, pipettor, 50mL centrifuge tubes, zirconium oxide beads (1.0mm or 2.0mm) or stainless steel beads (3.2mm ) .

Instructions:
  1. Cut liver into appropriately sized pieces for analysis (0.1g – 3g) and place into a 50mL centrifuge tube. NOTE: If you are using whole liver, you may have to remove the outer membrane to ensure proper homogenization.
  2. OPTIONAL: If desired, wash the tissue 3x with 5mL PBS to remove blood and loose connective tissue from the tissue
  3. Add a mass of beads based on your mass of sample – about 2x for zirconium oxide (1.0mm or 2.0mm) beads, or about 3x for stainless steel (3.2mm) beads.
  4. Add two volumes of buffer per mass of tissue (2mL of buffer per gram of tissue)
  5. Tightly screw the centrifuge tubes closed.
  6. Place tubes into the Bullet Blender™ 50.
  7. Set controls for SPEED 8 and TIME 3 minutes. Press start.
  8. After the run, remove the tubes from the instrument.
  9. Visually inspect samples, if homogenization is unsatisfactory, run for another three minutes at SPEED 10.
  10. Proceed with your downstream application.
SAFETY NOTE!!!

When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced.