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Innovative Lab Products for the Life Sciences

The Bullet Blender™ and Bullet Blender™ Blue

Your Meconium samples stay cool
during homogenization!

The Bullet Blender™ Lyse E. coli and other cells. Homogenize or disrupt tissue. The Bullet Blender is quiet and user friendly.
> No Cross Contamination
> Samples stay cool
> Convenient
> Risk  Free
> Established protocols

Homogenize up to 24 meconium samples simultaneously-- in less than 3 minutes!

 

The Bullet Blender™ enables you to homogenize up to 24 samples (meconium or just about any animal or plant tissue) in separate microcentrifuge tubes at a time.  Load up to 300mg meconium with your application buffer and grinding beads in standard polypropylene microcentrifuge tubes.  Place the tubes into the Bullet Blender™ (pictured above) or Bullet Blender™ Blue.  The "bullets" in the "blender" vigorously strike all the sample tubes as the instrument runs for a few minutes.  The agitation of the grinding agents with the tissues homogenizes the samples.

The  Bullet Blender™  and Bullet Blender™ Blue enable you to disrupt or homogenize up to 24 meconium or tissue/cell samples at a time.  Load the samples in standard polypropylene microcentrifuge tubes into the Bullet Blender ™.  Balls (the "bullets") repeatedly strike the sample tubes in a controlled manner, thousands of times per minutes, inducing vigorous motion of the tissue samples or other substances inside the tubes, providing efficient mixing. With beads in the tubes, the samples are thoroughly homogenized. Tune the extent of disruption by adjusting the speed. Click here to see sample protocols.

> No Cross Contamination

The samples are homogenized (or mixed) non-invasively.  The sample tubes are kept closed during agitation, as the samples are processed.  There are no probes to clean between samples.

> Samples Stay Cool

The instrument uses very little power due to the unique, patented design.  It uses a small DC motor to agitate the individual tubes, not ultrasonics.   Also, because the motor does not need to agitate any heavy platforms or plates, it is small and will last for years.

> Convenient to Use

Simply place your meconium or other tissue samples or cell cultures along with some beads in standard high quality polypropylene tubes, and load the tubes into the Bullet Blender™.   Set the duration (typically a few minutes) and speed (vigorousness).  There are no probes to clean.  And it is quieter than a sonicator and does not heat up your samples more than a few degrees!

> Risk Free Purchase

The Bullet Blender™ comes with a 30-day money back guarantee and a two year warranty, with a three year warranty on the motor.  The simple, reliable design enables the Bullet Blenders™ to sell for a fraction  of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.

> Established Protocols


Protocol for Meconium Homogenization in the Bullet Blender™

The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of human meconium. This protocol was developed for the extraction and downstream analysis of various chemical analytes. You may need to alter this protocol to adapt it to your downstream application.

Materials Required:

meconium, Bullet Blender™, methanol, pipettor, 2.0 ml microcentrifuge tubes, bead spatula, and 0.5mm stainless steel beads.

Instructions:
  1. Accurately weigh 0.25 g of meconium and add to a 2.0 ml microcentrifuge tube. Try to keep the walls of the tube as clean as possible, and ensure the meconium sits as low in the tube as possible.
  2. Add 1.4 ml of methanol to every tube.
  3. Add one scoop (~0.07 g) of 0.5mm stainless steel beads to each tube.
  4. Securely close the microcentrifuge tubes.
  5. Place tubes into the Bullet Blender™.
  6. Set controls for SPEED 8 and TIME 5 minutes. Press Start.
  7. After the run, remove tubes from the instrument.
  8. Verify that all samples have a uniform appearance. A smooth semi-liquid should exist. If homogenization is unsatisfactory, run for another five minutes at SPEED 8.
  9. Proceed with your downstream application.
SAFETY NOTE!!!

When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced.

Acknowledgment:
Thanks to Chantry Clark, Stephanie Marin, and ARUP labs for sharing this protocol!

Bullet Blender™ 05* Homogenization Protocol for Meconium

The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of human meconium. This protocol was developed for the extraction and downstream analysis of various chemical analytes. You may need to alter this protocol to adapt it to your downstream application.

Materials Required:

meconium, Bullet Blender™, methanol, pipettor, 5.0 ml centrifuge tubes, bead spatula, and 0.9-2.0 mm stainless steel bead blend.

Instructions:
  1. Accurately weigh 1.0 g of meconium and add to a 5.0 ml centrifuge tube. Try to keep the walls of the tube as clean as possible, and ensure the meconium sits as low in the tube as possible.
  2. Add 1.5 g (! 1/16 tsp.) of 0.9-2.0 mm stainless steel bead blend to every tube.
  3. Centrifuge all samples briefly to force the meconium and beads to the bottom of the tubes.
  4. Add 2.9 ml of methanol to every tube.
  5. Securely close the microcentrifuge tubes.
  6. Place tubes into the Bullet Blender™ 05.
  7. Set controls for SPEED 7 and TIME 5 minutes. Press Start.
  8. After the run, remove tubes from the instrument.
  9. Verify that all samples have a uniform appearance. A smooth semi-liquid should exist. If homogenization is unsatisfactory, run for another 3-5 minutes at SPEED 7.
  10. Proceed with your downstream application.
SAFETY NOTE!!!

When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced.

Acknowledgment:
Thanks to Chantry Clark, Stephanie Marin, and ARUP labs for sharing this protocol!

 

*The Bullet Blender 05 is a special-order unit meant for homogenizing volumes of tissue up to about 1 ml while providing more homogenization power than the Bullet Blender 50. If you think you would be interested in the Bullet Blender 05, please send an e-mail to sales@nextadvance.com.

The Bullet Blender™ home page shows the entire family of products and links to other applications.