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No Cross Contamination
The samples are homogenized (or mixed) non-invasively. The
sample tubes are kept closed during agitation, as the
samples are processed. There are no probes to
clean between samples.
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Samples Stay
Cool
The instrument uses very
little power due to the unique, patented design.
It uses a small DC motor to agitate the individual
tubes, not ultrasonics or larger rotors which generate a lot of heat. Also, because
the motor does not need to agitate any heavy
platforms or plates, it is small and will last for
years.
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Convenient to Use
Simply place your pharynx (oropharynx, nasopharynx, and / or laryngopharynx) and other tissue samples or cell cultures and some beads in standard
high quality polypropylene tubes, and load the tubes into the
Bullet Blender™. Set the duration (typically a few
minutes) and speed, then you're ready to homogenize. There are no probes to clean, it is quieter than a sonicator and does not heat up
your samples more than a few degrees!
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Risk Free Purchase
The Bullet Blender™
comes with a 30-day money back guarantee and a two year
warranty, with a three year warranty on the motor. The simple, reliable design enables the
Bullet Blenders™ to
sell for a fraction of the price of ultrasonic or
other agitation based instruments, yet provides an easier,
quicker technique.
> Established Protocols
Protocol for Pharyngeal Tissue Homogenization in the
Bullet Blender™
The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of Pharyngeal tissue. This protocol was created using oropharyngeal tissue from humans. Other types of pharyngeal tissue or pharyngeal tissue from other species may require a slightly modified homogenization protocol. This protocol does specify a buffer, and there are additives to assist in tissue disruption, however it is likely that tissue could be homogenized without enzymatic or chemical assistance if the homogenization time was increased.
Materials Required:
Pharyngeal tissue, Bullet Blender™, homogenization buffer, pipettor, microcentrifuge tubes, and 0.5.mm stainless steel beads (part number SSB05) or stainless steel bead blend (part number SSB14B).
Instructions:
- If larger than approximately 100mg, cut tissue into pieces or approximately 100mg or smaller.
- Place tissue into microcentrifuge tubes. No more than 300mg of tissue should be placed in a single tube.
- Add stainless steel beads to the tubes. Use 3 times the mass of the sample (approx. ½ the volume of the sample) in beads.
- Add digestion buffer (10mM Tris, pH 7.5; 10mM EDTA; 0.5% SDS; 200 mg/ml Proteinase K). Add two volumes of digestion buffer for each mass of tissue (for example, with 100mg tissue, use 200 ml buffer)
- Close the microcentrifuge tubes tightly, and place the tubes into the Bullet Blender™.
- Set controls for SPEED 10 and TIME 5 minutes. Press start.
- After the run, remove tubes from the instrument.
- Visually inspect samples, if homogenization is unsatisfactory, run for another three minutes at SPEED 10.
- Proceed with your downstream application.
SAFETY NOTE!!!
When using a centrifuge to separate your homogenate from
the debris and beads, make sure your tubes are balanced.
Reference:
Winder, D.M., Ball, S.L.R., Vaughan, K., Hanna, N., Woo, Y.L., Fränzer, J., Sterling, J.C., Stanley, M.A., Sudhoff, H., Goon, P.K.C. Sensitive HPV detection in oropharyngeal cancers. BMC Cancer 9(440): 2009
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