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Innovative Lab Products for the Life Sciences

The Bullet Blender™ and Bullet Blender™ Blue

Dig 'em up, blend 'em up, & analyze them!

The easiest and coolest way to harvest DNA, RNA and proteins from roots

No chemical lysis or harsh detergents required!
Your root, tuber, and rhizome samples stay cool!

The Bullet Blender™Lyse E. coli and other cells. Homogenize or disrupt tissue. The Bullet Blender is quiet and user friendly.
> No Cross Contamination
> Samples stay cool
> Convenient
> Risk  Free
> Established protocols

Homogenize up to 24 horseradish, malanga, ginger, or other samples simultaneously - in less than 3 minutes!

 

The Bullet Blender™ enables you to homogenize up to 24 samples in separate microcentrifuge tubes at a time.  Load up to 300mg root samples with your application buffer and grinding beads in standard polypropylene microcentrifuge tubes.  Place the tubes into the Bullet Blender™ (pictured above) or Bullet Blender™ Blue.  The "bullets" in the "blender" vigorously strike all the sample tubes as the instrument runs for a few minutes.  The agitation of the grinding agents with the tissues homogenizes the samples.

The Bullet Blender™ and Bullet Blender™ Blue enable you to disrupt or homogenize up to 24 root, rhizome, tuber, and other tissue/cell samples at a time.  Load the samples in standard polypropylene microcentrifuge tubes into the Bullet Blender ™.  Balls (the "bullets") repeatedly strike the sample tubes in a controlled manner, thousands of times per minutes, inducing vigorous motion of the root samples or other substances inside the tubes, providing efficient mixing. With beads in the tubes, the samples are thoroughly homogenized. Tune the extent of disruption by adjusting the speed. Click here to see sample protocols.

> No Cross Contamination

The samples are homogenized (or mixed) non-invasively.  The sample tubes are kept closed during agitation, as the samples are processed.  There are no probes to clean between samples.

> Samples Stay Cool

The instrument uses very little power due to the unique, patented design.  It uses a small DC motor to agitate the individual tubes, not ultrasonics.   Also, because the motor does not need to agitate any heavy platforms or plates, it is small and will last for years.

> Convenient to Use

Simply place your root and other plant samples or cell cultures along with some beads in standard high quality polypropylene tubes, and load the tubes into the Bullet Blender™.   Set the duration (typically a few minutes) and speed (vigorousness).  There are no probes to clean, it is quieter than a sonicator, and it does not heat up your samples more than a few degrees!

> Risk Free Purchase

The Bullet Blender™ comes with a 30-day money back guarantee and a two year warranty, with a three year warranty on the motor.  The simple, reliable design enables the Bullet Blender™ to sell for a fraction  of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.

> Established Protocols

See the protocols below for Ginger, Horseradish, and Malanga homogenization.

Protocol for Ginger Rhizome Homogenization in the Bullet Blender

The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of ginger rhizome (Zingiber officinale). This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.).

Materials Required:

ginger rhizome, Bullet Blender™, homogenization buffer, pipettor, microcentrifuge tubes, and 0.9-2.0mm stainless steel bead blend (part number SSB14B) or 1.0mm zirconium oxide beads (part number ZROB10).

Instructions:
  1. OPTIONAL: Wash ginger 3x with ~1mL PBS or water to remove soil and other surface debris.
  2. Cut ginger into long, thin slices of 200mg or less and place each slice into a microcentrifuge tube.
  3. Add a mass of stainless steel bead blend equal to 3x the mass of ginger, or a mass of zirconium oxide beads equal to 2x the mass of ginger.
  4. Close the microcentrifuge tubes and place them into the Bullet Blender™. NOTE:
    There should be no buffer in the tubes at this point.
  5. Set controls for SPEED 8 and TIME 4 and run the Bullet Blender™.
  6. Remove the samples from the Bullet Blender. The ginger should be coarsely pulverized. If not, run for another three minutes at speed 10.
  7. Add 2 volumes of buffer to the tube for every mass of sample (ex. for 100 mg ginger, add 0.2mL buffer)
  8. Close the microcentrifuge tubes and place them back into the Bullet Blender™.
  9. Set controls for SPEED 8 and TIME 3 minutes. Press Start.
  10. After the run, remove tubes from the instrument.
  11. Visually inspect samples. If homogenization is unsatisfactory, run for another three minutes at speed 10.
  12. Proceed with your downstream application.


Protocol for Horseradish Root Homogenization in the Bullet Blender

The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of horseradish (Armoracia rusticana) root. This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.).

Materials Required:

Horseradish root, Bullet Blender™, homogenization buffer, pipettor, microcentrifuge tubes, and 0.9-2.0mm stainless steel bead blend (part number SSB14B).

Instructions:
  1. OPTIONAL: Wash horseradish 3x with ~1mL PBS or water to remove soil and other surface debris.
  2. Cut horseradish into long, thin slices of 200mg or less and place each slice into a microcentrifuge tube.
  3. Add a mass of stainless steel bead blend equal to 3x the mass of horseradish, or a mass of zirconium oxide beads equal to 2x the mass of horseradish.
  4. Close the microcentrifuge tubes and place them into the Bullet Blender™. NOTE: There should be no buffer in the tubes at this point.
  5. Set controls for SPEED 10 and TIME 5 and run the Bullet Blender™.
  6. Remove the samples from the Bullet Blender. The horseradish should be coarsely pulverized. If not, run for another three minutes at speed 10.
  7. Add 2 volumes of buffer to the tube for every mass of sample (ex. for 100 mg horseradish, add 0.2mL buffer)
  8. Close the microcentrifuge tubes and place them back into the Bullet Blender™.
  9. Set controls for SPEED 8 and TIME 3 minutes. Press Start.
  10. After the run, remove tubes from the instrument.
  11. Visually inspect samples. If homogenization is unsatisfactory, run for another three minutes at speed 10.
  12. Proceed with your downstream application.


Protocol for Malanaga Tuber Homogenization in the Bullet Blender

The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of malanga (Xanthosoma sagittifolium) tuber. This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.).

Materials Required:

Malanga tuber, Bullet Blender™, homogenization buffer, pipettor, microcentrifuge tubes, and 0.9-2.0mm stainless steel bead blend (part number SSB14B).

Instructions:
  1. OPTIONAL: Wash malanga 3x with ~1mL PBS or water to remove soil and other surface debris.
  2. Cut malanga into long, thin slices of 200mg or less and place each slice into a microcentrifuge tube.
  3. Add a mass of stainless steel bead blend equal to 3x the mass of malanga, or a mass of zirconium oxide beads equal to 2x the mass of malanga.
  4. Close the microcentrifuge tubes and place them into the Bullet Blender™. NOTE: There should be no buffer in the tubes at this point.
  5. Set controls for SPEED 8 and TIME 4 and run the Bullet Blender™.
  6. Remove the samples from the Bullet Blender. The malanga should be coarsely pulverized. If not, run for another three minutes at speed 10.
  7. Add 2 volumes of buffer to the tube for every mass of sample (ex. for 100 mg malanga add 0.2mL buffer)
  8. Close the microcentrifuge tubes and place them back into the Bullet Blender™.
  9. Set controls for SPEED 8 and TIME 3 minutes. Press Start.
  10. After the run, remove tubes from the instrument.
  11. Visually inspect samples. If homogenization is unsatisfactory, run for another three minutes at speed 10.
  12. Proceed with your downstream application.

SAFETY NOTE!!!

When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced.

The Bullet Blender™ home page shows the entire family of products and links to other applications.