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Innovative Lab Products for the Life Sciences

The Bullet Blender™ and Bullet Blender™ Blue

Homogenizing sugar cane? Sweet!

The easiest and coolest way to harvest DNA, RNA and proteins from sugar cane

No chemical lysis or detergents required!
Your sugar cane samples stay cool!

The Bullet Blender™Lyse E. coli and other cells. Homogenize or disrupt tissue. The Bullet Blender is quiet and user friendly.
> No Cross Contamination
> Samples stay cool
> Convenient
> Risk  Free
> Established protocols

Homogenize up to 24 sugar cane or other samples simultaneously - in less than 3 minutes!

 

The Bullet Blender™ enables you to homogenize up to 24 samples in separate microcentrifuge tubes at a time.  Load up to 300mg sugar cane pulp samples (from the softer inner core of the sugar cane) with your application buffer and grinding beads in standard polypropylene microcentrifuge tubes.  Place the tubes into the Bullet Blender™ (pictured above) or Bullet Blender™ Blue.  The "bullets" in the "blender" vigorously strike all the sample tubes as the instrument runs for a few minutes.  The agitation of the grinding agents with the tissues homogenizes the samples.

The Bullet Blender™ and Bullet Blender™ Blue enable you to disrupt or homogenize up to 24 saccharum and other plant/tissue/cell samples at a time.  Load the samples in standard polypropylene microcentrifuge tubes into the Bullet Blender ™.  Balls (the "bullets") repeatedly strike the sample tubes in a controlled manner, thousands of times per minutes, inducing vigorous motion of the sugar samples or other substances inside the tubes, providing efficient mixing. With beads in the tubes, the samples are thoroughly homogenized. Tune the extent of disruption by adjusting the speed. Click here to see sample protocols.

> No Cross Contamination

The samples are homogenized (or mixed) non-invasively.  The sample tubes are kept closed during agitation, as the samples are processed.  There are no probes to clean between samples.

> Samples Stay Cool

The instrument uses very little power due to the unique, patented design.  It uses a small DC motor to agitate the individual tubes, not ultrasonics.   Also, because the motor does not need to agitate any heavy platforms or plates, it is small and will last for years.

> Convenient to Use

Simply place your root and other plant samples or cell cultures along with some beads in standard high quality polypropylene tubes, and load the tubes into the Bullet Blender™.   Set the duration (typically a few minutes) and speed (vigorousness).  There are no probes to clean, it is quieter than a sonicator, and it does not heat up your samples more than a few degrees!

> Risk Free Purchase

The Bullet Blender™ comes with a 30-day money back guarantee and a two year warranty, with a three year warranty on the motor.  The simple, reliable design enables the Bullet Blender™ to sell for a fraction  of the price of ultrasonic or other agitation based instruments, yet provides an easier, quicker technique.

> Established Protocols

See the protocol below for Sugar Cane homogenization.

Protocol for Sugar Cane Homogenization in the Bullet Blender

The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of the pulpous matter in the center of sugar canes (plants of the genus Saccharum). Please be aware that the highly fibrous outer sections of the cane are extremely tough and will be extremely difficult to homogenize. This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.).

Materials Required:

Sugar cane, Bullet Blender™, homogenization buffer, pipettor, microcentrifuge tubes, and 1.0mm zirconium oxide beads or 0.9- 2.0mm stainless steel bead blend (part number ZrOB10 or SSB14B).

Instructions:
  1. Place up to 300mg of sugar cane pulp into microcentrifuge tubes.
  2. Add a mass of the zirconium oxide beads equal to 3x the mass of the cane pulp or add stainless steel bead blend equal to 4x the mass of cane pulp. As an approximation, the beads should occupy about ½ the volume of the leaf.
  3. Add 2 volumes of buffer to the tube for every mass of sample (ex. for 100 mg sugar cane, add 200mL buffer)
  4. Close the microcentrifuge tubes and place them into the Bullet Blender™.
  5. Set controls for SPEED 9 and TIME 3 minutes. Press Start.
  6. After the run, remove tubes from the instrument.
  7. Visually inspect samples. If homogenization is unsatisfactory, run for another two minutes at speed 10.
  8. Proceed with your downstream application.


SAFETY NOTE!!!

When using a centrifuge to separate your homogenate from the debris and beads, make sure your tubes are balanced.

The Bullet Blender™ home page shows the entire family of products and links to other applications.