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The easiest and
coolest
way to harvest DNA, RNA and proteins from tumors
Your tumor samples stay cool! |
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The Bullet Blender™
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No Cross
Contamination
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Samples stay
cool
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Convenient
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Risk Free
Homogenize up to 24 tumor samples simultaneously, in just minutes!
The
Bullet Blender™ enables
you to homogenize up to 24 tumor or other samples in microcentrifuge
tubes
at a
time. Load up to .25g of tissue, along with
some beads and buffer, in standard polypropylene tubes into the
Bullet Blender™
(above) or the
Bullet Blender™ Blue.
The "bullets" in the "blender" vigorously strike all
the sample tubes simultaneously for a few minutes.
The agitation homogenizes the samples.
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RT-PCR results of
RNA from an epithelial carcinoma
homogenized with the Bullet Blender™.
Control transcript GAPDH (glyceraldehyde 3-phosphate dehydrogenase)
does not fluctuate while the transcript of interest
varies between samples. High quality RNA is
isolated, shown by A260/280 and A260/230
near 2.0.
Data provided by
Dr. Denis Schewe |
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“I can count on it for fast
consistent results.”
Dr. Denis
Schewe, Mt. Sinai Medical Center, New York City |
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The
Bullet Blender™ and
the Bullet Blender™ Blue
enable you to disrupt or homogenize up to 24 tumor and other tissue/cell
samples at a time. Load the samples in
standard polypropylene microcentrifuge tubes into the Bullet
Blender™.
Balls (the "bullets") repeatedly strike the sample
tubes in a controlled manner, thousands of times per
minutes, inducing vigorous motion of the plant
samples or other substances
inside the tubes, providing efficient
mixing. With beads in the tubes, the samples are
thoroughly homogenized. Tune the
extent of disruption by adjusting the speed.
Click here to see sample protocols. |
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No Cross Contamination
The samples are homogenized (or mixed) non-invasively. The
sample tubes are kept closed during agitation, as the
samples are processed. There are no probes to
clean between samples.
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Samples Stay
Cool
The instrument uses very
little power due to the unique, patented design.
It uses a small DC motor to agitate the individual
tubes, not ultrasonics. Also, because
the motor does not need to agitate any heavy
platforms or plates, it is small and will last for
years.
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Convenient to Use
Simply place your tumors and other tissue samples or cell cultures and some beads in standard
high quality polypropylene tubes, and load the tubes into the
Bullet Blender™.
Set the duration (typically
a few
minutes) and speed (vigorousness). There are no probes to clean.
And it is quieter than a sonicator and does not heat up
your samples more than a few degrees!
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Risk Free Purchase
The
Bullet Blender™ comes with a 30 day money back guarantee and a 2 year
warranty, with a 3 year warranty on the motor. The simple, reliable design enables the
Bullet
Blenders™ to
sell for a fraction of the price of ultrasonic or
other agitation based instruments, yet provides an easier,
quicker technique.
Click here to go to the main
Bullet Blender™ web page. |
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Protocol for
tumor tissue RNA extraction
Prepare one
microcentrifuge tube with 5 stainless steel beads
(about 1 scoop, 1.6mm diameter beads) for each
sample.
Add 1mL Trizol®
into each tube, followed by the tumor sample
(approximately 4mm x 4mm x 4mm).
Process immediately
in the Bullet Blender, speed 10 for 5 minutes.
If appearance of the sample homogenate is not
satisfactory, run for another 5 minutes.
Proceed with
manufaturer's protocol for Trizol® RNA extraction. |
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Protocol for Tumor Homogenization in the Bullet Blender™ The protocol described in this document is for the use of the Bullet Blender™ for the homogenization of tumor / cancer tissue (from a variety of animals). This protocol was developed using carcinoma. Note that due to the highly varied nature of tumors, especially tumors arising from different tissues, you may need to modify this protocol suit your specific needs. This protocol does not specify a particular buffer - you may choose which is most appropriate for your downstream application (nucleic acid isolation, protein extraction, etc.). Materials Required: tumor, buffer, Bullet Blender™, microcentrifuge tubes, 1.6mm stainless steel beads ( part number SSB16) , and pipettor. Instructions: - Cut tumor tissue into appropriately sized pieces for analysis (250mg or less) and place into a microcentrifuge tube.
- OPTIONAL: Wash tissue 3x with ~1mL PBS. Aspirate. NOTE: This step removes external contaminants (blood, etc.).
- Add a mass of 1.6mm stainless steel beads equal to 1.3 times your mass of tissue (so if you had 100mg of tissue, you would use 130mg of beads). One bead weighs 17mg.
- Add 3 volumes of buffer for every mass of tissue (so for 100mg of tissue, you would add 300ml of buffer).
- Close the microcentrifuge tubes.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 10 and TIME 5 minutes. Press Start.
- After the run, remove tubes from the instrument.
- Inspect samples. If homogenization is unsatisfactory, run for another five minutes at SPEED 10.
- Proceed with your downstream application.
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