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No Cross Contamination
The samples are homogenized non-invasively. The
sample tubes are kept closed during agitation, as the
samples are processed. There are no probes to
clean between samples.
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Samples Stay
Cool
The instrument uses very
little power due to the unique, patented design.
It uses a small DC motor to agitate the individual
tubes, not ultrasonics. Also, because
the motor does not need to agitate any heavy
platforms or plates, it is small and will last for
years.
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Convenient to Use
Simply place your uterine tissue and other tissue samples or cell cultures and some beads in standard
high quality polypropylene tubes, and load the tubes into the
Bullet Blender™. Set the duration (typically a few
minutes) and speed (vigorousness). There are no probes to clean.
And it is quieter than a sonicator and does not heat up
your samples more than a few degrees!
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Risk Free Purchase
The Bullet Blender™
comes with a 30-day money back guarantee and a two year
warranty, with a three year warranty on the motor. The simple, reliable design enables the
Bullet Blenders™ to
sell for a fraction of the price of ultrasonic or
other agitation based instruments, yet provides an easier,
quicker technique.
> Established Protocols
Protocol for Uterine (Uterus) Tissue Homogenization in the Bullet Blender™
The protocol described in this document is for the use of the Bullet Blender™ for the
homogenization of uterine tissue / myocardium (from a variety of animals). Note that the
time and speed settings may differ due to the variation in consistency / texture of cardiac
tissue from species to species. This protocol does not specify a particular buffer - you may
choose which is most appropriate for your downstream application (nucleic acid isolation,
protein extraction, etc.).
Materials Required:
uterine tissue, saline, aspirator, Bullet Blender™, microcentrifuge
tubes, stainless steel beads (1.6mm, product number SSB16 or
0.9-2.0mm blend, product number SSB14B), homogenization
buffer, and pipettor.
Instructions:
- Cut uterine tissue into appropriately sized pieces for analysis (50mg-150mg) and place into a
microcentrifuge tube.
- OPTIONAL: Wash tissue 3x with ~1mL PBS. Aspirate. NOTE: This step removes
some external contaminants (blood, etc.).
- Add stainless steel beads (1.6mm OR 0.9mm-2.0mm blend) to the tube. Use a
mass of beads equal to 3x the mass of tissue. One scoop of stainless steel blend ≈ 220mg. One scoop of 1.6mm stainless steel beads ≈ 186mg.
- Add 2 volumes of buffer for every mass of tissue,
minimum of 25μL.
- Close the microcentrifuge tubes.
- Place tubes into the Bullet Blender™.
- Set controls for SPEED 8 and TIME 5 minutes. Press Start.
- After the run, remove tubes from the instrument.
- Visually inspect samples. If homogenization is unsatisfactory, run for another three
minutes at speed 10.
- Proceed with your downstream application.
SAFETY NOTE!!!
When using a centrifuge to separate your homogenate from
the debris and beads, make sure your tubes are balanced.
Typical Results
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