Get Better LC/MS Results
. . . and Save Money Too!
Pack your own HPLC
capillary columns.
Kasil/formamide frit kit included.
Load your samples into mass
spectrometers without transfer loss or contact with
metallic surfaces.
High Quality
Reliable
High Value
Risk Free
Left: High Pressure model.
Center-front: standard model.
Right: standard model with integrated magnetic stirplate.
Kasil/formamide
frit kit
Included with all our pressure cells. With Kasil 1 and Kasil 1624
The FRIT-KIT, $45, includes: 1.0mL Kasil 1, 1.5mL Kasil
1624, 0.5mL formamide,
and a ceramic cleaving tool to cut the fused silica
capillaries.
Transparent Capillary Loader Look inside while packing your column!
The PC1000, $1395, has a
transparent body, so you can see the level of packing
suspension in the vial. Rated for 1000 psi - sufficient for most column packing applications.
“The pressure injection cell is really
handy. I have two in my lab, one for packing capillaries
and one for loading samples in my mass spec. Packing
my own capillaries saves me hundreds of
dollars on each one.”
Dr. Qishan Lin, Director of the
Proteomics Core Facility
The Center for Functional Genomics, University at Albany, New York
"We have been getting some very high quality results from the column packed by your pressure cell."
Prof. Austin Yang, The University of Maryland
New and improved PC77 Pressure
Injection Cell with wider base.
Rated for 2500 psi (17,000 kPa). Includes a
three-way valve for convenience. $1095
PC8500 Pressure Injection Cell. High pressure version of
PC77, rated for 8500 psi (58,000 kPa). $1595
PC77-MAG Pressure injection cell with integrated
magnetic stirplate, for convenience and perfect alignment,
and 5x2mm stirbar.
$1495
Orders include an operator's manual, a hex wrench, and 10
additional PTFE ferrules (for 360 to 400um outside
diameter capillaries). Accessories such as tubing,
capillary, and frit assemblies are available.
(Prices do not include shipping or taxes)
Column Packing Hardware Kits. We also stock
column packing kits which include what you need in
addition to the pressure injection cell to pack your own
capillary columns. Each kit includes: HPREG, a high
pressure regulator (up to 1500 psi); TBNG10, 10 feet of
1/8 inch diameter stainless steel tubing to connect the
cell to the gas cylinder (or other pressure source);
CAP-75 10 meters of 75 um inside diameter fused silica capillary
and a cleaving tool; and STRBR5X2 small magnetic, Teflon
coated (5 x 2
mm) stir bar.
We also carry stainless steel tubing, fused silica
capillary, pressure regulators, adapters, and other items you may need with your
capillary loader.
"Hey, it works great. I am getting much better resolution
since packing my own columns and using the cell to
directly inject my samples. The first purchase in a long
time that was well worth the money.
It is well made and so easy to use. After I purchased the
fittings to tie it all together, it’s a snap!"
Dr. Steve Mouton, Northrop
Grumman Proteomics, Texas
"We’re saving tons of money packing our own columns. It works great and it’s very user friendly."
Dr. Kimberly McKinney, Carolinas Medical Center, North Carolina
The Pressure Injection
Cell, sometimes called a “bomb loader” enables
controlled dispensing of small-volume liquid
samples. Using high pressure, the
pressure injection cell has proved
invaluable for two applications: densely packing nanobore capillary columns with solid-phase
particles; and precisely infusing microliter samples
directly from microcentrifuge tubes into mass
spectrometers without additional transfers, wasted
sample, or contact with metallic surfaces which
adsorb some negatively charged molecules such as
peptides with phospho or carboxy groups.
The
Pressure Injection Cell holds 1 mL and 2
mL micro-centrifuge tubes as well as 12 x 32 mm
glass vials in its central chamber (as shown at right). The assembly can be
used on a typical magnetic stirrer, e.g. to keep
particles in suspension. The cell needs to be
connected to a bottle/tank of compressed gas, such
as Helium or Nitrogen. Packing capillary columns
requires several hundred to about 1000 psi or
sometimes more, and mass spectrometer injections
typically require a few hundred psi. This unit
is rated for a 2500 psi. 8500 psi units are
available.
The Pressure Injection Cells accept various sample
tubes, including 0.5 mL, 1.5 mL, and 2.0 mL microcentrifuge tubes and 12 mm diameter glass
vials.
Why
pay hundreds of dollars per packed capillary column
for HPLC? You can pack the columns, of
various internal diameters, yourself.
The Pressure Injection Cell comes with instructions
on how to pack columns and is available as a stand
alone unit (with 9 extra ferrules), or as a
complete kit with ferrules, 1/8"
stainless steel tubing, a spool
of capillary, a cleaving tool, and a frit assembly,
or as a partial kit.
With the Pressure
Injection Cell, load your sample into a mass
spectrometer directly from a microcentrifuge or
Eppendorf tube
and avoid transfer loss. There is no contact with
metallic surfaces which would adsorb negatively
charged molecules.
The Pressure Injection Cell is
made with the finest methods and components, thereby
enabling you to acquire very accurate data.
The high quality stainless steel fittings and valve
are made by Swagelok. The body and cap
of the pressure chamber are nickel coated.
Special features not found elsewhere include a
recess in the cap to allow easy access to
the sample tube and a hexagonal shape to help align
the cap to the body.
The simple and
reliable product design, efficient manufacturing,
internet marketing, and our low overhead enable us
to sell the Pressure Injection Cells at a price
almost 50% less than competitors charge.
We don't skimp on quality; we make the best product
available. However, we do pass the savings
along to our customers.
The Pressure
Injection Cell comes with a 30 day money back
guarantee and a 2 year warranty.
Features
Easy sample tube removal
since the tube protrudes above the bottom section of the
pressure injection cell.
O-ring seal at interface
ensures no leakage.
Rated
for 2500 psi (17,000 kPa), ideal for consistent results with bottled gas.
Available in
8500 psi (56,000 kPa) version
Includes a 3-way valve for easy switching between
pressurization and release of pressure
3 bolts tightly clamp the
top section to the bottom.
Holds 0.5 to 2 ml
microcentrifuge/Eppendorf tubes and 12 x 32 mm (glass)
vials.
Includes
operator's manual; 10 reusable ferrules for typical
(380 µm outside diameter) capillaries (other ferrules
are available); and 1.5 mL of Kasil and 0.5 mL of
formamide and a cleaving tool for making your own frits.
Available with integrated magnetic stirplate.
Warranty: 2 years parts and labor.
30 day money back guarantee.
Operation
Operationally,
the pressure injection cell is analogous to a straw in a
juice box. As you apply pressure in the box,
the juice will be forced out through the straw.
Likewise,
the pressure injection cell is connected to pressurized
gas, typically in a tank, as shown on the far right.
A 0.5 to 2 mL tube containing the sample liquid is placed in
the base of the pressure injection cell, as shown
schematically on the left (bolts are not shown). A
capillary is then placed through the ferrule in the cap
and down into the sample tube. When packing a
capillary, a frit assembly is placed at the distal end of
the capillary to prevent particles from exiting and the
entire pressure injection cell is typically placed on a
stir plate (as shown above). By regulating the gas
pressure, you can adjust the flow rate of the sample into
the capillary.
Sample Data
LC/MS chromatogram of a peptide mixture prepared from a
tryptic digested gel band, separated by a 5 µm C18 resin
packed column (100 µm inside diameter by 10 cm long
capillary). The Pressure Injection Cell was used
to pack the HPLC capillary column and to inject the sample
into the mass spectrometer. Courtesy of Dr. Qishan
Lin.
SCHEMATIC OF SYSTEM WITH
PRESSURE INJECTION CELL
Accessories:
TBNG10 $103, Tubing
Use this 10 foot long piece of stainless
steel tubing, 1/8 inch outside diameter, .028 wall, rated
for 8,500 psi, to connect the pressure injection
cell to the pressure regulator on the gas tank.
HPREG $550 High pressure regulator mounts to an Argon, Helium, or Nitrogen gas
tank, and comes with a Swagelok fitting for 1/8 inch diameter tubing.
Operating pressure (reaching the pressure injection cell) of up to 1500 psi. To
use this regulator with air, order the inlet nipple for air, NIP-AIR below.
Temporarily just $150/spool, because we have too much
inventory.
CAP-75 $150. Fused Silica Capillary, 75 µm ID and cleaving tool.
This is the most popular size of capillary used for making
packed columns. The 10 meter length of capillary comes on a spool and a
cleaving tool is included. This flexible, synthetic fused silica capillary has a
polyimide coating and is 100% proof tested at 100 ksi.
ADPT-ISOto1/8
$21 Adapter which threads into 1/4 ISO fitting (metric) on the pressure
regulator and accepts 1/8 inch tubing (such as TBNG10 above) from the pressure
injection cell.
ADPT-3mm1/8 $27 Adapter which mounts on the PC77 and accepts 3mm (metric) outside diameter
tubing. This item will come assembled to the pressure injection cell.
ADPT-1/4to1/8
$15 Adapter which connects 1/8 inch outside diameter
tubing to a fitting for 1/4 tubing.
NIP-AIR
$35 Inlet Nipple and Nut to adapt HPREG above (high pressure regulator) for use
with tanks of dry air. Order with the HPREG and it will be factory
installed with the regulator.
STRBR5X2 $8 Magnetic stirbar, Teflon® coated, 5 x 2 mm.
Likewise,
the pressure injection cell is connected to pressurized
gas, typically in a tank, as shown on the far right.
A 0.5 to 2 mL tube containing the sample liquid is placed in
the base of the pressure injection cell, as shown
schematically on the left (bolts are not shown). A
capillary is then placed through the ferrule in the cap
and down into the sample tube. When packing a
capillary, a frit assembly is placed at the distal end of
the capillary to prevent particles from exiting and the
entire pressure injection cell is typically placed on a
stir plate (as shown above). By regulating the gas
pressure, you can adjust the flow rate of the sample into
the capillary.
