Bullet Blender for Sample Lysis
The Bullet Blender bead mill homogenizer can efficiently prepare up to 24 samples in just minutes. Using patented striking technology, it thoroughly lyses samples, ensuring they are ready for downstream applications. Each sample is homogenized consistently, resulting in high yields and minimal variation between samples. For detailed preparation times and speeds, visit the protocols page.
PrecisionPak™ for Genomic DNA Extraction
The Next Advance Bullet Blender paired with the PrecisionPak™ streamlines the homogenization and extraction workflow, delivering excellent results. Various packs are available, each with protocols tailored for specific tissue types and biomolecule extraction. These kits include Proteinase K and RNase I to effectively remove RNA molecules, and FoamBlocker to minimize foaming of the homogenate. Each kit also provides an optimized protocol tailored to your specific tissue.
For over a dozen years, the bead lysis kits in the PrecisionPak have been trusted and cited by researchers worldwide. These kits include a combination of bead sizes to ensure thorough tissue homogenization without degrading nucleic acids. Simply add your sample to the lysis kit tube and homogenize at the appropriate settings for optimal results. For details protocols and settings, visit our protocols page.
Step 2: DNA Extraction
Our DNA extraction kits use advanced magnetic bead technology instead of traditional spin columns to achieve optimal results. By mixing magnetic beads with the homogenate, the DNA has extended contact time with the active surface. As a result, this method reduces high shear stresses on genomic DNA (gDNA) that would occur during centrifugation with spin columns. Furthermore, it cuts the processing time and effort in half. The Magnetic Rack Assembly also simplifies the extraction process, streamlining the workflow. Additionally, our kits are free from organic solvents, ensuring a safer and more environmentally friendly approach. The quality of the extracted DNA is suitable for PCR amplification, Next-Generation Sequencing (NGS), micro-array genotyping, and enzymatic reactions.
Comparison of PrecisionPak MAGneat Tissue DNA Extraction Kit and “Qompetitor” Kit. Purified DNA from 30mg of various tissue samples were extracted either using PrecisionPak MAGneat Tissue DNA Extraction kit or “Qompetitor” kit, following manufacturer’s instructions. Extracted DNA samples were run on a 1% Agarose gel. M – 1 kb ladder.
PCR Amplification from DNA Isolated Using PrecisionPak MAGneat Animal Tissue DNA Extraction kit. PCR amplification was performed to amplify a 900bp fragment of ACT1 gene. PCR products were run on a 1% Agarose gel. M – 100bp ladder.
