Feathers (vanes and down) Bullet Blender® homogenization protocol
Extract molecules (DNA, RNA, protein, chemicals)
Dry final product
Sample sizes: 10 to 100 mg.
Notes on the protocol: None.
Materials Required
uL
One of these Bullet Blenders
Bullet Blender 5E (BBY5E)
Bullet Blender 5 Gold (BB5E-AU)
Reagents
None required
Bead choices
2.0 mm zirconium oxide beads (ZROB20) use 500ul beads
Procedure
Place the sample in the tube with the beads.
Close the tubes tightly and place them in the Bullet Blender.
Set the controls for Speed 20 and Time 10. Press Start.
After the run, remove the tubes from the instrument and visually inspect the samples. If homogenization is incomplete, homogenize for another 5 minutes.