Ideal for Adipose Tissue Homogenization
Do you spend lots of time and effort homogenizing adipose tissue samples? The Bullet Blender® tissue homogenizer delivers high quality and superior yields. No other homogenizer comes close to delivering the Bullet Blender’s winning combination of top-quality performance and budget-friendly affordability. See below for a adipose tissue homogenization protocol.
Save Time, Effort and Get Superior Results with
The Bullet Blender Homogenizer
Consistent and High Yield Results
Run up to 24 samples at the same time under microprocessor-controlled conditions, ensuring experimental reproducibility and high yield. Process samples from 10mg or less up to 3.5g.No Cross Contamination
No part of the Bullet Blender ever touches the tissue – the sample tubes are kept closed during homogenization. There are no probes to clean between samples.Samples Stay Cool
The Bullet Blenders’ innovative and elegant design provides convective cooling of the samples, so they do not heat up more than several degrees. In fact, our Gold+ models hold the sample temperature to about 4ºC.Easy and Convenient to Use
Just place beads and buffer along with your tissue sample in standard tubes, load tubes directly in the Bullet Blender, select time and speed, and press start.Risk Free Purchase
Thousands of peer-reviewed journal articles attest to the consistency and quality of the Bullet Blender homogenizer. We offer a 2 year warranty, extendable to 4 years, because our Bullet Blenders are reliable and last for many years.Adipose Tissue Homogenization Protocol
Sample size |
See the Protocol |
| microcentrifuge tube model (up to 300 mg) | Small adipose tissue samples |
| 5mL tube model (100mg - 1g) | Medium adipose tissue samples |
| 50mL tube model (100mg - 3.5g) | Large adipose tissue samples |
What Else Can You Homogenize? Tough or Soft, No Problem!Â
The Bullet Blender can process a wide range of samples including organ tissue, cell culture, plant tissue, and small organisms. You can homogenize samples as tough as mouse femur or for gentle applications such as tissue dissociation or organelle isolation.
Adipose tissue pieces (on beads in upper photo) are completely homogenized into the buffer (slightly darker or discolored in lower photo).
Want more guidance? Need a quote? Contact us:
Bullet Blender Models
Select Publications using the Bullet Blender to Homogenize Adipose Tissue
Albury-Warren, T. M., Pandey, V., Spinel, L. P., Masternak, M., & Altomare, D. A. (2015). Prediabetes linked to excess glucagon in transgenic mice with pancreatic active AKT1. Journal of Endocrinology, JOE-15-0288. https://doi.org/10.1530/JOE-15-0288
Neve, E. P. A., Köfeler, H., Hendriks, D. F. G., Nordling, Å., Gogvadze, V., Mkrtchian, S., Näslund, E., & Ingelman-Sundberg, M. (2015). Expression and Function of mARC: Roles in Lipogenesis and Metabolic Activation of Ximelagatran. PLOS ONE, 10(9), e0138487. https://doi.org/10.1371/journal.pone.0138487
Keung, E. Z., Akdemir, K. C., Al Sannaa, G. A., Garnett, J., Lev, D., Torres, K. E., Lazar, A. J., Rai, K., & Chin, L. (2015). Increased H3K9me3 drives dedifferentiated phenotype via KLF6 repression in liposarcoma. Journal of Clinical Investigation, 125(8), 2965–2978. https://doi.org/10.1172/JCI77976
Dordevic, A., Pendergast, F., Morgan, H., Villas-Boas, S., Caldow, M., Larsen, A., Sinclair, A., & Cameron-Smith, D. (2015). Postprandial Responses to Lipid and Carbohydrate Ingestion in Repeated Subcutaneous Adipose Tissue Biopsies in Healthy Adults. Nutrients, 7(7), 5347–5361. https://doi.org/10.3390/nu7075224
Liu, L., Zou, P., Zheng, L., Linarelli, L. E., Amarell, S., Passaro, A., Liu, D., & Cheng, Z. (2015). Tamoxifen reduces fat mass by boosting reactive oxygen species. Cell Death and Disease, 6(1), e1586. https://doi.org/10.1038/cddis.2014.553
White, P. J., Mitchell, P. L., Schwab, M., Trottier, J., Kang, J. X., Barbier, O., & Marette, A. (2015). Transgenic ω-3 PUFA enrichment alters morphology and gene expression profile in adipose tissue of obese mice: Potential role for protectins. Metabolism, 64(6), 666–676. https://doi.org/10.1016/j.metabol.2015.01.017
Fukunaga, T., Zou, W., Rohatgi, N., Colca, J. R., & Teitelbaum, S. L. (2015). An Insulin-Sensitizing Thiazolidinedione, Which Minimally Activates PPARγ, Does Not Cause Bone Loss: EFFECT OF AN INSULIN-SENSITIZING TZD ON BONE LOSS. Journal of Bone and Mineral Research, 30(3), 481–488. https://doi.org/10.1002/jbmr.2364
Do, A., Menon, V., Zhi, X., Gesing, A., Wiesenborn, D., Spong, A., Sun, L., Bartke, A., & Masternak, M. M. (2015). Thyroxine modifies the effects of growth hormone in Ames dwarf mice. Aging (Albany, NY), 7(4), 241–255. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429089/
Kahraman, S., Dirice, E., De Jesus, D. F., Hu, J., & Kulkarni, R. N. (2014). Maternal insulin resistance and transient hyperglycemia impact the metabolic and endocrine phenotypes of offspring. AJP: Endocrinology and Metabolism, 307(10), E906–E918. https://doi.org/10.1152/ajpendo.00210.2014
Sutton, A. K., Pei, H., Burnett, K. H., Myers, M. G., Rhodes, C. J., & Olson, D. P. (2014). Control of Food Intake and Energy Expenditure by Nos1 Neurons of the Paraventricular Hypothalamus. Journal of Neuroscience, 34(46), 15306–15318. https://doi.org/10.1523/JNEUROSCI.0226-14.2014
Goetzman, E. S., Alcorn, J. F., Bharathi, S. S., Uppala, R., McHugh, K. J., Kosmider, B., Chen, R., Zuo, Y. Y., Beck, M. E., McKinney, R. W., Skilling, H., Suhrie, K. R., Karunanidhi, A., Yeasted, R., Otsubo, C., Ellis, B., Tyurina, Y. Y., Kagan, V. E., Mallampalli, R. K., & Vockley, J. (2014). Long-chain Acyl-CoA Dehydrogenase Deficiency as a Cause of Pulmonary Surfactant Dysfunction. Journal of Biological Chemistry, 289(15), 10668–10679. https://doi.org/10.1074/jbc.M113.540260
Hindle, A. G., & Martin, S. L. (2014). Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation. AJP: Endocrinology and Metabolism, 306(3), E284–E299. https://doi.org/10.1152/ajpendo.00431.2013
Menon, V., Zhi, X., Hossain, T., Bartke, A., Spong, A., Gesing, A., & Masternak, M. M. (2014). The contribution of visceral fat to improved insulin signaling in Ames dwarf mice. Aging Cell, 13(3), 497–506. https://doi.org/10.1111/acel.12201
Bajpai, G., Simmen, R. C. M., & Stenken, J. A. (2014). In vivo microdialysis sampling of adipokines CCL2, IL-6, and leptin in the mammary fat pad of adult female rats. Mol. BioSyst., 10(4), 806–812. https://doi.org/10.1039/C3MB70308H
Melero, M., García-Párraga, D., Corpa, J., Ortega, J., Rubio-Guerri, C., Crespo, J., Rivera-Arroyo, B., & Sánchez-Vizcaíno, J. (2014). First molecular detection and characterization of herpesvirus and poxvirus in a Pacific walrus (Odobenus rosmarus divergens). BMC Veterinary Research, 10(1), 968. https://doi.org/10.1186/s12917-014-0308-2
Vaughan, C. H., Zarebidaki, E., Ehlen, J. C., & Bartness, T. J. (2014). Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue. In Methods in Enzymology (Vol. 537, pp. 199–225). Elsevier. http://linkinghub.elsevier.com/retrieve/pii/B9780124116191000112
Cheng, X., Guo, S., Liu, Y., Chu, H., Hakimi, P., Berger, N. A., Hanson, R. W., & Kao, H.-Y. (2013). Ablation of Promyelocytic Leukemia Protein (PML) Re-patterns Energy Balance and Protects Mice from Obesity Induced by a Western Diet. Journal of Biological Chemistry, 288(41), 29746–29759. https://doi.org/10.1074/jbc.M113.487595
Lee, T.-W. A., Kwon, H., Zong, H., Yamada, E., Vatish, M., Pessin, J. E., & Bastie, C. C. (2013). Fyn Deficiency Promotes a Preferential Increase in Subcutaneous Adipose Tissue Mass and Decreased Visceral Adipose Tissue Inflammation. Diabetes, 62(5), 1537–1546. https://doi.org/10.2337/db12-0920
Liu, B.-H., Lin, Y.-Y., Wang, Y.-C., Huang, C.-W., Chen, C.-C., Wu, S.-C., Mersmann, H. J., Cheng, W. T. K., & Ding, S.-T. (2013). Porcine Adiponectin Receptor 1 Transgene Resists High-fat/Sucrose Diet-Induced Weight Gain, Hepatosteatosis and Insulin Resistance in Mice. Experimental Animals, 62(4), 347–360. https://doi.org/10.1538/expanim.62.347
Chaker, B., Samra, T. A., Datta, N. S., & Abou-Samra, A. B. (2013). Altered Responses to Cold Environment in Urocortin 1 and Corticotropin-Releasing Factor Deficient Mice. Physiology Journal, 2013, 1–7. https://doi.org/10.1155/2013/185767
Kumar, M., Roe, K., Nerurkar, P. V., Namekar, M., Orillo, B., Verma, S., & Nerurkar, V. R. (2012). Impaired Virus Clearance, Compromised Immune Response and Increased Mortality in Type 2 Diabetic Mice Infected with West Nile Virus. PLoS ONE, 7(8), e44682. https://doi.org/10.1371/journal.pone.0044682
Bastie, C. C., Gaffney-Stomberg, E., Lee, T.-W. A., Dhima, E., Pessin, J. E., & Augenlicht, L. H. (2012). Dietary Cholecalciferol and Calcium Levels in a Western-Style Defined Rodent Diet Alter Energy Metabolism and Inflammatory Responses in Mice. Journal of Nutrition, 142(5), 859–865. https://doi.org/10.3945/jn.111.149914
Carlstrom, M., Larsen, F. J., Nystrom, T., Hezel, M., Borniquel, S., Weitzberg, E., & Lundberg, J. O. (2010). Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice. Proceedings of the National Academy of Sciences, 107(41), 17716–17720. https://doi.org/10.1073/pnas.1008872107
