Liver Tissue Homogenizer & Homogenization Protocol

Ideal for Liver Tissue Homogenization

Do you spend lots of time and effort homogenizing Liver tissue samples? The Bullet Blender® tissue homogenizer delivers high quality and superior yields. No other homogenizer comes close to delivering the Bullet Blender’s winning combination of top-quality performance and budget-friendly affordability. See below for a Liver tissue homogenization protocol.

Save Time, Effort and Get Superior Results with

The Bullet Blender Homogenizer

Consistent and High Yield Results

Run up to 24 samples at the same time under microprocessor-controlled conditions, ensuring experimental reproducibility and high yield. Process samples from 10mg or less up to 3.5g.

No Cross Contamination

No part of the Bullet Blender ever touches the tissue – the sample tubes are kept closed during homogenization. There are no probes to clean between samples.

Samples Stay Cool

The Bullet Blenders’ innovative and elegant design provides convective cooling of the samples, so they do not heat up more than several degrees. In fact, our Gold+ models hold the sample temperature to about 4ºC.

Easy and Convenient to Use

Just place beads and buffer along with your tissue sample in standard tubes, load tubes directly in the Bullet Blender, select time and speed, and press start.

Risk Free Purchase

Thousands of peer-reviewed journal articles attest to the consistency and quality of the Bullet Blender homogenizer. We offer a 2 year warranty, extendable to 4 years, because our Bullet Blenders are reliable and last for many years.  

Liver Tissue Homogenization Protocol

Sample size

See the Protocol
microcentrifuge tube model (up to 300 mg) Small liver samples
5mL tube model (100mg - 1g) Medium liver samples
50mL tube model (100mg - 3.5g) Large liver samples

What Else Can You Homogenize? Tough or Soft, No Problem! 

The Bullet Blender can process a wide range of samples including organ tissue, cell culture, plant tissue, and small organisms. You can homogenize samples as tough as mouse femur or for gentle applications such as tissue dissociation or organelle isolation.

the Bullet Blender high-throughput tissue homogenizer

Liver tissue pieces (on beads in upper photo) are completely homogenized into the buffer (slightly darker in lower photo). 

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    Bullet Blender Models

    Select Publications using the Bullet Blender to Homogenize Liver Tissue

    See all of our Bullet Blender publications!

    Kim, J. M., Park, S. K., Guo, T. J., Kang, J. Y., Ha, J. S., Lee, D. S., Lee, U., & Heo, H. J. (2016). Anti-amnesic effect of Dendropanax morbifera via JNK signaling pathway on cognitive dysfunction in high-fat diet-induced diabetic mice. Behavioural Brain Research, 312, 39–54. https://doi.org/10.1016/j.bbr.2016.06.013
    Krutzke, L., Prill, J. M., Engler, T., Schmidt, C. Q., Xu, Z., Byrnes, A. P., Simmet, T., & Kreppel, F. (2016). Substitution of blood coagulation factor X-binding to Ad5 by position-specific PEGylation: Preventing vector clearance and preserving infectivity. Journal of Controlled Release, 235, 379–392. https://doi.org/10.1016/j.jconrel.2016.06.022
    Shreeram, S., Ramesh, S., Puthan, J. K., Balakrishnan, G., Subramanian, R., Reddy, M. T., & Pereira, S. L. (2016). Age associated decline in the conversion of leucine to β-Hydroxy-β-Methylbutyrate in rats. Experimental Gerontology, 80, 6–11. https://doi.org/10.1016/j.exger.2016.03.021
    Gunderson, M. P., Pickett, M. A., Martin, J. T., Hulse, E. J., Smith, S. S., Smith, L. A., Campbell, R. M., Lowers, R. H., Boggs, A. S. P., & Guillette Jr., L. J. (2016). Variations in hepatic biomarkers in American alligators (Alligator mississippiensis) from three sites in Florida, USA. Chemosphere, 155, 180–187. https://doi.org/10.1016/j.chemosphere.2016.04.018
    Miranda, C. L., Elias, V. D., Hay, J. J., Choi, J., Reed, R. L., & Stevens, J. F. (2016). Xanthohumol improves dysfunctional glucose and lipid metabolism in diet-induced obese C57BL/6J mice. Archives of Biochemistry and Biophysics, 599, 22–30. https://doi.org/10.1016/j.abb.2016.03.008
    Wang, Y., & Pfeiffer, J. K. (2016). Emergence of a Large-Plaque Variant in Mice Infected with Coxsackievirus B3. MBio, 7(2), e00119-16. https://doi.org/10.1128/mBio.00119-16
    Li, M., Li, C., Song, S., Kang, H., Yang, D., & Li, G. (2016). Development and antigenic characterization of three recombinant proteins with potential for Glässer’s disease prevention. Vaccine, 34(19), 2251–2258. https://doi.org/10.1016/j.vaccine.2016.03.014
    Johns, M., Esmaeili Mohsen Abadi, S., Malik, N., Lee, J., Neumann, W. L., Rausaria, S., Imani-Nejad, M., McPherson, T., Schober, J., & Kwon, G. (2016). Oral administration of SR-110, a peroxynitrite decomposing catalyst, enhances glucose homeostasis, insulin signaling, and islet architecture in B6D2F1 mice fed a high fat diet. Archives of Biochemistry and Biophysics, 596, 126–137. https://doi.org/10.1016/j.abb.2016.03.002
    Bosch, M., Fajardo, A., Alcalá-Vida, R., Fernández-Vidal, A., Tebar, F., Enrich, C., Cardellach, F., Pérez-Navarro, E., & Pol, A. (2016). Hepatic Primary and Secondary Cholesterol Deposition and Damage in Niemann-Pick Disease. The American Journal of Pathology, 186(3), 517–523. https://doi.org/10.1016/j.ajpath.2015.12.002
    Adamo, R. F., Fishbein, I., Zhang, K., Wen, J., Levy, R. J., Alferiev, I. S., & Chorny, M. (2016). Magnetically enhanced cell delivery for accelerating recovery of the endothelium in injured arteries. Journal of Controlled Release, 222, 169–175. https://doi.org/10.1016/j.jconrel.2015.12.025
    Lee, L.-C., Wei, L., Huang, W.-C., Hsu, Y.-J., Chen, Y.-M., & Huang, C.-C. (2015). Hypolipidemic Effect of Tomato Juice in Hamsters in High Cholesterol Diet-Induced Hyperlipidemia. Nutrients, 7(12), 10525–10537. https://doi.org/10.3390/nu7125552
    Hajkova, K., Jurasek, B., Sykora, D., Palenicek, T., Miksatkova, P., & Kuchar, M. (2015). Salting-out-assisted liquid–liquid extraction as a suitable approach for determination of methoxetamine in large sets of tissue samples. Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-015-9221-1
    Mattsson, A., Kärrman, A., Pinto, R., & Brunström, B. (2015). Metabolic Profiling of Chicken Embryos Exposed to Perfluorooctanoic Acid (PFOA) and Agonists to Peroxisome Proliferator-Activated Receptors. PLOS ONE, 10(12), e0143780. https://doi.org/10.1371/journal.pone.0143780
    Hernandez-Anzaldo, S., Berry, E., Brglez, V., Leung, D., Yun, T. J., Lee, J. S., Filep, J. G., Kassiri, Z., Cheong, C., Lambeau, G., Lehner, R., & Fernandez-Patron, C. (2015). Identification of a Novel Heart-Liver Axis: Matrix Metalloproteinase-2 Negatively Regulates Cardiac Secreted Phospholipase A2 to Modulate Lipid Metabolism and Inflammation in the Liver. Journal of the American Heart Association, 4(11), e002553–e002553. https://doi.org/10.1161/JAHA.115.002553
    Falendysz, E. A., Lopera, J. G., Lorenzsonn, F., Salzer, J. S., Hutson, C. L., Doty, J., Gallardo-Romero, N., Carroll, D. S., Osorio, J. E., & Rocke, T. E. (2015). Further Assessment of Monkeypox Virus Infection in Gambian Pouched Rats (Cricetomys gambianus) Using In Vivo Bioluminescent Imaging. PLOS Neglected Tropical Diseases, 9(10), e0004130. https://doi.org/10.1371/journal.pntd.0004130
    Wagschal, A., Najafi-Shoushtari, S. H., Wang, L., Goedeke, L., Sinha, S., deLemos, A. S., Black, J. C., Ramírez, C. M., Li, Y., Tewhey, R., Hatoum, I., Shah, N., Lu, Y., Kristo, F., Psychogios, N., Vrbanac, V., Lu, Y.-C., Hla, T., de Cabo, R., … Näär, A. M. (2015). Genome-wide identification of microRNAs regulating cholesterol and triglyceride homeostasis. Nature Medicine, 21(11), 1290–1297. https://doi.org/10.1038/nm.3980
    Albury-Warren, T. M., Pandey, V., Spinel, L. P., Masternak, M., & Altomare, D. A. (2015). Prediabetes linked to excess glucagon in transgenic mice with pancreatic active AKT1. Journal of Endocrinology, JOE-15-0288. https://doi.org/10.1530/JOE-15-0288
    McKenna, S., Gossling, M., Bugarini, A., Hill, E., Anderson, A. L., Rancourt, R. C., Balasubramaniyan, N., El Kasmi, K. C., & Wright, C. J. (2015). Endotoxemia Induces I B /NF- B-Dependent Endothelin-1 Expression in Hepatic Macrophages. The Journal of Immunology, 195(8), 3866–3879. https://doi.org/10.4049/jimmunol.1501017
    Goedeke, L., Rotllan, N., Canfrán-Duque, A., Aranda, J. F., Ramírez, C. M., Araldi, E., Lin, C.-S., Anderson, N. N., Wagschal, A., de Cabo, R., Horton, J. D., Lasunción, M. A., Näär, A. M., Suárez, Y., & Fernández-Hernando, C. (2015). MicroRNA-148a regulates LDL receptor and ABCA1 expression to control circulating lipoprotein levels. Nature Medicine, 21(11), 1280–1289. https://doi.org/10.1038/nm.3949
    Neve, E. P. A., Köfeler, H., Hendriks, D. F. G., Nordling, Å., Gogvadze, V., Mkrtchian, S., Näslund, E., & Ingelman-Sundberg, M. (2015). Expression and Function of mARC: Roles in Lipogenesis and Metabolic Activation of Ximelagatran. PLOS ONE, 10(9), e0138487. https://doi.org/10.1371/journal.pone.0138487
    Peleli, M., Hezel, M., Zollbrecht, C., Persson, A. E. G., Lundberg, J. O., Weitzberg, E., Fredholm, B. B., & Carlström, M. (2015). In adenosine A2B knockouts acute treatment with inorganic nitrate improves glucose disposal, oxidative stress, and AMPK signaling in the liver. Frontiers in Physiology, 6. https://doi.org/10.3389/fphys.2015.00222
    Wang, Z., Neal, B. H., Lamb, J. C., & Klaunig, J. E. (2015). Mechanistic Investigation of Toxaphene Induced Mouse Liver Tumors. Toxicological Sciences, kfv151. https://doi.org/10.1093/toxsci/kfv151
    Ran, L., Yu, Q., Zhang, S., Xiong, F., Cheng, J., Yang, P., Xu, J.-F., Nie, H., Zhong, Q., Yang, X., Yang, F., Gong, Q., Kuczma, M., Kraj, P., Gu, W., Ren, B.-X., & Wang, C.-Y. (2015). Cx3cr1 deficiency in mice attenuates hepatic granuloma formation during acute schistosomiasis by enhancing the M2-type polarization of macrophages. Disease Models & Mechanisms, 8(7), 691–700. https://doi.org/10.1242/dmm.018242
    Danan-Gotthold, M., Golan-Gerstl, R., Eisenberg, E., Meir, K., Karni, R., & Levanon, E. Y. (2015). Identification of recurrent regulated alternative splicing events across human solid tumors. Nucleic Acids Research, 43(10), 5130–5144. https://doi.org/10.1093/nar/gkv210
    Kollitz, E. M., Zhang, G., Hawkins, M. B., Whitfield, G. K., Reif, D. M., & Kullman, S. W. (2015). Molecular Cloning, Functional Characterization, and Evolutionary Analysis of Vitamin D Receptors Isolated from Basal Vertebrates. PLOS ONE, 10(4), e0122853. https://doi.org/10.1371/journal.pone.0122853
    Gao, X., Yang, T., Liu, M., Peleli, M., Zollbrecht, C., Weitzberg, E., Lundberg, J. O., Persson, A. E. G., & Carlstrom, M. (2015). NADPH Oxidase in the Renal Microvasculature Is a Primary Target for Blood Pressure-Lowering Effects by Inorganic Nitrate and Nitrite. Hypertension, 65(1), 161–170. https://doi.org/10.1161/HYPERTENSIONAHA.114.04222
    Techer, D., Milla, S., Fontaine, P., Viot, S., & Thomas, M. (2015). Influence of waterborne gallic and pelargonic acid exposures on biochemical and reproductive parameters in the zebrafish ( Danio rerio ): Influence Of Gallic and Pelargonic Acid Exposure. Environmental Toxicology, n/a-n/a. https://doi.org/10.1002/tox.22228
    Doldur-Balli, F., Ozel, M. N., Gulsuner, S., Tekinay, A. B., Ozcelik, T., Konu, O., & Adams, M. M. (2015). Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ). BMC Neuroscience, 16(1). https://doi.org/10.1186/s12868-015-0229-4
    Guard, J., Sanchez-Ingunza, R., Shah, D. H., Rothrock, M. J., Gast, R. K., & Jones, D. R. (2015). Recovery of Salmonella enterica serovar Enteritidis from hens initially infected with serovar Kentucky. Food Chemistry, 189, 86–92. https://doi.org/10.1016/j.foodchem.2015.02.018
    Parafati, M., Lascala, A., Morittu, V. M., Trimboli, F., Rizzuto, A., Brunelli, E., Coscarelli, F., Costa, N., Britti, D., Ehrlich, J., Isidoro, C., Mollace, V., & Janda, E. (2015). Bergamot polyphenol fraction prevents nonalcoholic fatty liver disease via stimulation of lipophagy in cafeteria diet-induced rat model of metabolic syndrome. The Journal of Nutritional Biochemistry, 26(9), 938–948. https://doi.org/10.1016/j.jnutbio.2015.03.008
    Hezel, M. P., Liu, M., Schiffer, T. A., Larsen, F. J., Checa, A., Wheelock, C. E., Carlström, M., Lundberg, J. O., & Weitzberg, E. (2015). Effects of long-term dietary nitrate supplementation in mice. Redox Biology, 5, 234–242. https://doi.org/10.1016/j.redox.2015.05.004
    Wang, Y.-M., Chai, S. C., Lin, W., Chai, X., Elias, A., Wu, J., Ong, S. S., Pondugula, S. R., Beard, J. A., Schuetz, E. G., Zeng, S., Xie, W., & Chen, T. (2015). Serine 350 of human pregnane X receptor is crucial for its heterodimerization with retinoid X receptor alpha and transactivation of target genes in vitro and in vivo. Biochemical Pharmacology, 96(4), 357–368. https://doi.org/10.1016/j.bcp.2015.06.018
    Neishabouri, S. H., Hutson, S. M., & Davoodi, J. (2015). Chronic activation of mTOR complex 1 by branched chain amino acids and organ hypertrophy. Amino Acids, 47(6), 1167–1182. https://doi.org/10.1007/s00726-015-1944-y
    Jandegian, C. M., Deem, S. L., Bhandari, R. K., Holliday, C. M., Nicks, D., Rosenfeld, C. S., Selcer, K. W., Tillitt, D. E., vom Saal, F. S., Vélez-Rivera, V., Yang, Y., & Holliday, D. K. (2015). Developmental exposure to bisphenol A (BPA) alters sexual differentiation in painted turtles (Chrysemys picta). General and Comparative Endocrinology, 216, 77–85. https://doi.org/10.1016/j.ygcen.2015.04.003
    Eriksson, A., Williams, M. J., Voisin, S., Hansson, I., Krishnan, A., Philippot, G., Yamskova, O., Herisson, F. M., Dnyansagar, R., Moschonis, G., Manios, Y., Chrousos, G. P., Olszewski, P. K., Frediksson, R., & Schiöth, H. B. (2015). Implication of coronin 7 in body weight regulation in humans, mice and flies. BMC Neuroscience, 16(1), 13. https://doi.org/10.1186/s12868-015-0151-9
    Zhang, Z., He, L., Hu, S., Wang, Y., Lai, Q., Yang, P., Yu, Q., Zhang, S., Xiong, F., Simsekyilmaz, S., Ning, Q., Li, J., Zhang, D., Zhang, H., Xiang, X., Zhou, Z., Sun, H., & Wang, C.-Y. (2015). AAL exacerbates pro-inflammatory response in macrophages by regulating Mincle/Syk/Card9 signaling along with the Nlrp3 inflammasome assembly. American Journal of Translational Research, 7(10), 1812–1825.
    Ali, K., Abo-Ali, E. M., Kabir, M. D., Riggins, B., Nguy, S., Li, L., Srivastava, U., & Thinn, S. M. M. (2014). A Western-Fed Diet Increases Plasma HDL and LDL-Cholesterol Levels in ApoD–/– Mice. PLoS ONE, 9(12), e115744. https://doi.org/10.1371/journal.pone.0115744
    Drake, J. C., Bruns, D. R., Peelor, F. F., Biela, L. M., Miller, R. A., Hamilton, K. L., & Miller, B. F. (2014). Long-lived crowded-litter mice have an age-dependent increase in protein synthesis to DNA synthesis ratio and mTORC1 substrate phosphorylation. AJP: Endocrinology and Metabolism, 307(9), E813–E821. https://doi.org/10.1152/ajpendo.00256.2014
    Hyatt, L. D., Wasserman, G. A., Rah, Y. J., Matsuura, K. Y., Coleman, F. T., Hilliard, K. L., Pepper-Cunningham, Z. A., Ieong, M., Stumpo, D. J., Blackshear, P. J., Quinton, L. J., Mizgerd, J. P., & Jones, M. R. (2014). Myeloid ZFP36L1 Does Not Regulate Inflammation or Host Defense in Mouse Models of Acute Bacterial Infection. PLoS ONE, 9(10), e109072. https://doi.org/10.1371/journal.pone.0109072
    Vernochet, C., Damilano, F., Mourier, A., Bezy, O., Mori, M. A., Smyth, G., Rosenzweig, A., Larsson, N.-G., & Kahn, C. R. (2014). Adipose tissue mitochondrial dysfunction triggers a lipodystrophic syndrome with insulin resistance, hepatosteatosis, and cardiovascular complications. The FASEB Journal, 28(10), 4408–4419. https://doi.org/10.1096/fj.14-253971
    Bannantine, J. P., Everman, J. L., Rose, S. J., Babrak, L., Katani, R., Barletta, R. G., Talaat, A. M., Grohn, Y. T., Chang, Y.-F., Kapur, V., & Bermudez, L. E. (2014). Evaluation of eight live attenuated vaccine candidates for protection against challenge with virulent Mycobacterium avium subspecies paratuberculosis in mice. Frontiers in Cellular and Infection Microbiology, 4. https://doi.org/10.3389/fcimb.2014.00088
    Zhu, L., Martinez, M. N., Emfinger, C. H., Palmisano, B. T., & Stafford, J. M. (2014). Estrogen signaling prevents diet-induced hepatic insulin resistance in male mice with obesity. AJP: Endocrinology and Metabolism, 306(10), E1188–E1197. https://doi.org/10.1152/ajpendo.00579.2013
    Goetzman, E. S., Alcorn, J. F., Bharathi, S. S., Uppala, R., McHugh, K. J., Kosmider, B., Chen, R., Zuo, Y. Y., Beck, M. E., McKinney, R. W., Skilling, H., Suhrie, K. R., Karunanidhi, A., Yeasted, R., Otsubo, C., Ellis, B., Tyurina, Y. Y., Kagan, V. E., Mallampalli, R. K., & Vockley, J. (2014). Long-chain Acyl-CoA Dehydrogenase Deficiency as a Cause of Pulmonary Surfactant Dysfunction. Journal of Biological Chemistry, 289(15), 10668–10679. https://doi.org/10.1074/jbc.M113.540260
    van der Plas-Duivesteijn, S. J., Mohammed, Y., Dalebout, H., Meijer, A., Botermans, A., Hoogendijk, J. L., Henneman, A. A., Deelder, A. M., Spaink, H. P., & Palmblad, M. (2014). Identifying Proteins in Zebrafish Embryos Using Spectral Libraries Generated from Dissected Adult Organs and Tissues. Journal of Proteome Research, 13(3), 1537–1544. https://doi.org/10.1021/pr4010585
    Offer, S. M., Butterfield, G. L., Jerde, C. R., Fossum, C. C., Wegner, N. J., & Diasio, R. B. (2014). microRNAs miR-27a and miR-27b Directly Regulate Liver Dihydropyrimidine Dehydrogenase Expression through Two Conserved Binding Sites. Molecular Cancer Therapeutics, 13(3), 742–751. https://doi.org/10.1158/1535-7163.MCT-13-0878
    Neely, C. J., Kartchner, L. B., Mendoza, A. E., Linz, B. M., Frelinger, J. A., Wolfgang, M. C., Maile, R., & Cairns, B. A. (2014). Flagellin Treatment Prevents Increased Susceptibility to Systemic Bacterial Infection after Injury by Inhibiting Anti-Inflammatory IL-10+ IL-12- Neutrophil Polarization. PLoS ONE, 9(1), e85623. https://doi.org/10.1371/journal.pone.0085623
    Baghdadi, H. (2014). Antioxidant Potential of Quercetin: Remarkable Protection against Hypercholesterolemia in Rats. British Journal of Medicine and Medical Research, 4(26), 4382–4391. https://doi.org/10.9734/BJMMR/2014/11126
    Borschensky, C. M., & Reinacher, M. (2014). Mutations in the 3c and 7b genes of feline coronavirus in spontaneously affected FIP cats. Research in Veterinary Science, 97(2), 333–340. https://doi.org/10.1016/j.rvsc.2014.07.016
    Penney, R., Lundgreen, A., Yao-Borengasser, A., Edavana, V., Williams, S., Dhakal, I., Wolff, R., Kadlubar, S., & Slattery, M. (2014). CYP19A1 single nucleotide polymorphism associations with CYP19A1, NFκB1, and IL6 gene expression in human normal colon and normal liver samples. Pharmacogenomics and Personalized Medicine, 163. https://doi.org/10.2147/PGPM.S62238
    Hill, A. J., Drever, N., Yin, H., Tamayo, E., Saade, G., & Bytautiene, E. (2014). The role of NADPH oxidase in a mouse model of fetal alcohol syndrome. American Journal of Obstetrics and Gynecology, 210(5), 466.e1-466.e5. https://doi.org/10.1016/j.ajog.2013.12.019

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