Lung Tissue Homogenizer & Homogenization Protocol

Ideal for Lung Tissue Homogenization

Do you spend lots of time and effort homogenizing Lung tissue samples? The Bullet Blender® tissue homogenizer delivers high quality and superior yields. No other homogenizer comes close to delivering the Bullet Blender’s winning combination of top-quality performance and budget-friendly affordability. See below for a Lung tissue homogenization protocol.

Save Time, Effort and Get Superior Results with

The Bullet Blender Homogenizer

Consistent and High Yield Results

Run up to 24 samples at the same time under microprocessor-controlled conditions, ensuring experimental reproducibility and high yield. Process samples from 10mg or less up to 3.5g.

No Cross Contamination

No part of the Bullet Blender ever touches the tissue – the sample tubes are kept closed during homogenization. There are no probes to clean between samples.

Samples Stay Cool

The Bullet Blenders’ innovative and elegant design provides convective cooling of the samples, so they do not heat up more than several degrees. In fact, our Gold+ models hold the sample temperature to about 4ºC.

Easy and Convenient to Use

Just place beads and buffer along with your tissue sample in standard tubes, load tubes directly in the Bullet Blender, select time and speed, and press start.

Risk Free Purchase

Thousands of peer-reviewed journal articles attest to the consistency and quality of the Bullet Blender homogenizer. We offer a 2 year warranty, extendable to 4 years, because our Bullet Blenders are reliable and last for many years.  

Lung Tissue Homogenization Protocol

Sample size

See the Protocol
microcentrifuge tube model (up to 300 mg) Small lung samples
5mL tube model (100mg - 1g) Medium lung samples
50mL tube model (100mg - 3.5g) Large lung samples

What Else Can You Homogenize? Tough or Soft, No Problem! 

The Bullet Blender can process a wide range of samples including organ tissue, cell culture, plant tissue, and small organisms. You can homogenize samples as tough as mouse femur or for gentle applications such as tissue dissociation or organelle isolation.

the Bullet Blender high-throughput tissue homogenizer

Lung tissue pieces (on beads in upper photo) are completely homogenized into the buffer (slightly darker in lower photo). 

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    Bullet Blender Models

    Select Publications using the Bullet Blender to Homogenize Lung Tissue

    See all of our Bullet Blender publications!

    Li, M., Li, C., Song, S., Kang, H., Yang, D., & Li, G. (2016). Development and antigenic characterization of three recombinant proteins with potential for Glässer’s disease prevention. Vaccine, 34(19), 2251–2258. https://doi.org/10.1016/j.vaccine.2016.03.014
    Adamo, R. F., Fishbein, I., Zhang, K., Wen, J., Levy, R. J., Alferiev, I. S., & Chorny, M. (2016). Magnetically enhanced cell delivery for accelerating recovery of the endothelium in injured arteries. Journal of Controlled Release, 222, 169–175. https://doi.org/10.1016/j.jconrel.2015.12.025
    Bawadekar, M., Gendron-Fitzpatrick, A., Rebernick, R., Shim, D., Warner, T. F., Nicholas, A. P., Lundblad, L. K. A., Thompson, P. R., & Shelef, M. A. (2016). Tumor necrosis factor alpha, citrullination, and peptidylarginine deiminase 4 in lung and joint inflammation. Arthritis Research & Therapy, 18, 173. https://doi.org/10.1186/s13075-016-1068-0
    Hajkova, K., Jurasek, B., Sykora, D., Palenicek, T., Miksatkova, P., & Kuchar, M. (2015). Salting-out-assisted liquid–liquid extraction as a suitable approach for determination of methoxetamine in large sets of tissue samples. Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-015-9221-1
    Ruhaak, L. R., Taylor, S. L., Stroble, C., Nguyen, U. T., Parker, E. A., Song, T., Lebrilla, C. B., Rom, W. N., Pass, H., Kim, K., Kelly, K., & Miyamoto, S. (2015). Differential N-Glycosylation Patterns in Lung Adenocarcinoma Tissue. Journal of Proteome Research, 14(11), 4538–4549. https://doi.org/10.1021/acs.jproteome.5b00255
    Falendysz, E. A., Lopera, J. G., Lorenzsonn, F., Salzer, J. S., Hutson, C. L., Doty, J., Gallardo-Romero, N., Carroll, D. S., Osorio, J. E., & Rocke, T. E. (2015). Further Assessment of Monkeypox Virus Infection in Gambian Pouched Rats (Cricetomys gambianus) Using In Vivo Bioluminescent Imaging. PLOS Neglected Tropical Diseases, 9(10), e0004130. https://doi.org/10.1371/journal.pntd.0004130
    McKenna, S., Gossling, M., Bugarini, A., Hill, E., Anderson, A. L., Rancourt, R. C., Balasubramaniyan, N., El Kasmi, K. C., & Wright, C. J. (2015). Endotoxemia Induces I B /NF- B-Dependent Endothelin-1 Expression in Hepatic Macrophages. The Journal of Immunology, 195(8), 3866–3879. https://doi.org/10.4049/jimmunol.1501017
    Marien, E., Meister, M., Muley, T., Fieuws, S., Bordel, S., Derua, R., Spraggins, J., Van de Plas, R., Dehairs, J., Wouters, J., Bagadi, M., Dienemann, H., Thomas, M., Schnabel, P. A., Caprioli, R. M., Waelkens, E., & Swinnen, J. V. (2015). Non-small cell lung cancer is characterized by dramatic changes in phospholipid profiles: Altered Phospholipid Profiles in NSCLC. International Journal of Cancer, 137(7), 1539–1548. https://doi.org/10.1002/ijc.29517
    Otsubo, C., Bharathi, S., Uppala, R., Ilkayeva, O. R., Wang, D., McHugh, K., Zou, Y., Wang, J., Alcorn, J. F., Zuo, Y. Y., Hirschey, M. D., & Goetzman, E. S. (2015). Long-chain Acylcarnitines Reduce Lung Function by Inhibiting Pulmonary Surfactant. Journal of Biological Chemistry, 290(39), 23897–23904. https://doi.org/10.1074/jbc.M115.655837
    Rosen, D. A., Hilliard, J. K., Tiemann, K. M., Todd, E. M., Morley, S. C., & Hunstad, D. A. (2015). Klebsiella pneumoniae FimK Promotes Virulence in Murine Pneumonia. Journal of Infectious Diseases, jiv440. https://doi.org/10.1093/infdis/jiv440
    Danan-Gotthold, M., Golan-Gerstl, R., Eisenberg, E., Meir, K., Karni, R., & Levanon, E. Y. (2015). Identification of recurrent regulated alternative splicing events across human solid tumors. Nucleic Acids Research, 43(10), 5130–5144. https://doi.org/10.1093/nar/gkv210
    Kim, B. S., Serebreni, L., Fallica, J., Hamdan, O., Wang, L., Johnston, L., Kolb, T., Damarla, M., Damico, R., & Hassoun, P. M. (2015). Cyclin-Dependent Kinase Five Mediates Activation of Lung Xanthine Oxidoreductase in Response to Hypoxia. PLOS ONE, 10(4), e0124189. https://doi.org/10.1371/journal.pone.0124189
    Caverly, L. J., Caceres, S. M., Fratelli, C., Happoldt, C., Kidwell, K. M., Malcolm, K. C., Nick, J. A., & Nichols, D. P. (2015). Mycobacterium abscessus Morphotype Comparison in a Murine Model. PLOS ONE, 10(2), e0117657. https://doi.org/10.1371/journal.pone.0117657
    Diaz, J., Warren, L., Helfner, L., Xue, X., Chatterjee, P. K., Gupta, M., Solanki, M. H., Esposito, M., Bonagura, V., & Metz, C. N. (2015). Obesity shifts house dust mite-induced airway cellular infiltration from eosinophils to macrophages: effects of glucocorticoid treatment. Immunologic Research, 63(1–3), 197–208. https://doi.org/10.1007/s12026-015-8717-2
    Khademi, S., Frye, M. A., Jeckel, K. M., Schroeder, T., Monnet, E., Irwin, D. C., Cole, P. A., Bell, C., Miller, B. F., & Hamilton, K. L. (2015). Hypoxia mediated pulmonary edema: potential influence of oxidative stress, sympathetic activation and cerebral blood flow. BMC Physiology, 15(1). https://doi.org/10.1186/s12899-015-0018-4
    Hilliard, K. L., Allen, E., Traber, K. E., Kim, Y., Wasserman, G. A., Jones, M. R., Mizgerd, J. P., & Quinton, L. J. (2015). Activation of Hepatic STAT3 Maintains Pulmonary Defense during Endotoxemia. Infection and Immunity, 83(10), 4015–4027. https://doi.org/10.1128/IAI.00464-15
    Tian, X., Liu, M., Su, X., Jiang, Z., Ma, Q., Liao, X., Li, X., Zhou, Z., Li, C., & Zhou, R. (2015). Mapping the epitope of neutralizing monoclonal antibodies against human adenovirus type 3. Virus Research, 208, 66–72. https://doi.org/10.1016/j.virusres.2015.06.002
    Lopera, J. G., Falendysz, E. A., Rocke, T. E., & Osorio, J. E. (2015). Attenuation of monkeypox virus by deletion of genomic regions. Virology, 475, 129–138. https://doi.org/10.1016/j.virol.2014.11.009
    Eriksson, A., Williams, M. J., Voisin, S., Hansson, I., Krishnan, A., Philippot, G., Yamskova, O., Herisson, F. M., Dnyansagar, R., Moschonis, G., Manios, Y., Chrousos, G. P., Olszewski, P. K., Frediksson, R., & Schiöth, H. B. (2015). Implication of coronin 7 in body weight regulation in humans, mice and flies. BMC Neuroscience, 16(1), 13. https://doi.org/10.1186/s12868-015-0151-9
    Chen, G.-S., Huang, K.-F., Huang, C.-C., & Wang, J.-Y. (2015). Thaliporphine Derivative Improves Acute Lung Injury after Traumatic Brain Injury. BioMed Research International, 2015, 1–10. https://doi.org/10.1155/2015/729831
    Zhang, Z., He, L., Hu, S., Wang, Y., Lai, Q., Yang, P., Yu, Q., Zhang, S., Xiong, F., Simsekyilmaz, S., Ning, Q., Li, J., Zhang, D., Zhang, H., Xiang, X., Zhou, Z., Sun, H., & Wang, C.-Y. (2015). AAL exacerbates pro-inflammatory response in macrophages by regulating Mincle/Syk/Card9 signaling along with the Nlrp3 inflammasome assembly. American Journal of Translational Research, 7(10), 1812–1825.
    Hyatt, L. D., Wasserman, G. A., Rah, Y. J., Matsuura, K. Y., Coleman, F. T., Hilliard, K. L., Pepper-Cunningham, Z. A., Ieong, M., Stumpo, D. J., Blackshear, P. J., Quinton, L. J., Mizgerd, J. P., & Jones, M. R. (2014). Myeloid ZFP36L1 Does Not Regulate Inflammation or Host Defense in Mouse Models of Acute Bacterial Infection. PLoS ONE, 9(10), e109072. https://doi.org/10.1371/journal.pone.0109072
    Rose, S. J., Neville, M. E., Gupta, R., & Bermudez, L. E. (2014). Delivery of Aerosolized Liposomal Amikacin as a Novel Approach for the Treatment of Nontuberculous Mycobacteria in an Experimental Model of Pulmonary Infection. PLoS ONE, 9(9), e108703. https://doi.org/10.1371/journal.pone.0108703
    Miller, M., Rosenthal, P., Beppu, A., Mueller, J. L., Hoffman, H. M., Tam, A. B., Doherty, T. A., McGeough, M. D., Pena, C. A., Suzukawa, M., Niwa, M., & Broide, D. H. (2014). ORMDL3 Transgenic Mice Have Increased Airway Remodeling and Airway Responsiveness Characteristic of Asthma. The Journal of Immunology, 192(8), 3475–3487. https://doi.org/10.4049/jimmunol.1303047
    Goetzman, E. S., Alcorn, J. F., Bharathi, S. S., Uppala, R., McHugh, K. J., Kosmider, B., Chen, R., Zuo, Y. Y., Beck, M. E., McKinney, R. W., Skilling, H., Suhrie, K. R., Karunanidhi, A., Yeasted, R., Otsubo, C., Ellis, B., Tyurina, Y. Y., Kagan, V. E., Mallampalli, R. K., & Vockley, J. (2014). Long-chain Acyl-CoA Dehydrogenase Deficiency as a Cause of Pulmonary Surfactant Dysfunction. Journal of Biological Chemistry, 289(15), 10668–10679. https://doi.org/10.1074/jbc.M113.540260
    Bailey, K. L., Bonasera, S. J., Wilderdyke, M., Hanisch, B. W., Pavlik, J. A., DeVasure, J., Robinson, J. E., Sisson, J. H., & Wyatt, T. A. (2014). Aging causes a slowing in ciliary beat frequency, mediated by PKC. AJP: Lung Cellular and Molecular Physiology, 306(6), L584–L589. https://doi.org/10.1152/ajplung.00175.2013
    Neely, C. J., Kartchner, L. B., Mendoza, A. E., Linz, B. M., Frelinger, J. A., Wolfgang, M. C., Maile, R., & Cairns, B. A. (2014). Flagellin Treatment Prevents Increased Susceptibility to Systemic Bacterial Infection after Injury by Inhibiting Anti-Inflammatory IL-10+ IL-12- Neutrophil Polarization. PLoS ONE, 9(1), e85623. https://doi.org/10.1371/journal.pone.0085623
    Borschensky, C. M., & Reinacher, M. (2014). Mutations in the 3c and 7b genes of feline coronavirus in spontaneously affected FIP cats. Research in Veterinary Science, 97(2), 333–340. https://doi.org/10.1016/j.rvsc.2014.07.016
    Goosen, W. J., Miller, M. A., Chegou, N. N., Cooper, D., Warren, R. M., van Helden, P. D., & Parsons, S. D. C. (2014). Agreement between assays of cell-mediated immunity utilizing Mycobacterium bovis-specific antigens for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer). Veterinary Immunology and Immunopathology, 160(1–2), 133–138. https://doi.org/10.1016/j.vetimm.2014.03.015
    Melero, M., García-Párraga, D., Corpa, J., Ortega, J., Rubio-Guerri, C., Crespo, J., Rivera-Arroyo, B., & Sánchez-Vizcaíno, J. (2014). First molecular detection and characterization of herpesvirus and poxvirus in a Pacific walrus (Odobenus rosmarus divergens). BMC Veterinary Research, 10(1), 968. https://doi.org/10.1186/s12917-014-0308-2
    Ivanov, S. S., & Roy, C. R. (2013). Pathogen signatures activate a ubiquitination pathway that modulates the function of the metabolic checkpoint kinase mTOR. Nature Immunology, 14(12), 1219–1228. https://doi.org/10.1038/ni.2740
    Yamamoto, K., Ahyi, A.-N. N., Pepper-Cunningham, Z. A., Ferrari, J. D., Wilson, A. A., Jones, M. R., Quinton, L. J., & Mizgerd, J. P. (2013). Roles of Lung Epithelium in Neutrophil Recruitment During Pneumococcal Pneumonia. American Journal of Respiratory Cell and Molecular Biology, 130906140418002. https://doi.org/10.1165/rcmb.2013-0114OC
    Karauzum, H., Adhikari, R. P., Sarwar, J., Devi, V. S., Abaandou, L., Haudenschild, C., Mahmoudieh, M., Boroun, A. R., Vu, H., Nguyen, T., Warfield, K. L., Shulenin, S., & Aman, M. J. (2013). Structurally Designed Attenuated Subunit Vaccines for S. aureus LukS-PV and LukF-PV Confer Protection in a Mouse Bacteremia Model. PLoS ONE, 8(6), e65384. https://doi.org/10.1371/journal.pone.0065384
    Chorny, M., Fishbein, I., Tengood, J. E., Adamo, R. F., Alferiev, I. S., & Levy, R. J. (2013). Site-specific gene delivery to stented arteries using magnetically guided zinc oleate-based nanoparticles loaded with adenoviral vectors. The FASEB Journal, 27(6), 2198–2206. https://doi.org/10.1096/fj.12-224659
    Nakayama, K. H., Lee, C. C. I., Batchelder, C. A., & Tarantal, A. F. (2013). Tissue Specificity of Decellularized Rhesus Monkey Kidney and Lung Scaffolds. PLoS ONE, 8(5), e64134. https://doi.org/10.1371/journal.pone.0064134
    Moghadaszadeh, B., Rider, B. E., Lawlor, M. W., Childers, M. K., Grange, R. W., Gupta, K., Boukedes, S. S., Owen, C. A., & Beggs, A. H. (2013). Selenoprotein N deficiency in mice is associated with abnormal lung development. The FASEB Journal, 27(4), 1585–1599. https://doi.org/10.1096/fj.12-212688
    Al-Hasan, Y. M., Evans, L. C., Pinkas, G. A., Dabkowski, E. R., Stanley, W. C., & Thompson, L. P. (2013). Chronic Hypoxia Impairs Cytochrome Oxidase Activity Via Oxidative Stress in Selected Fetal Guinea Pig Organs. Reproductive Sciences, 20(3), 299–307. https://doi.org/10.1177/1933719112453509
    Hoang, H. D., Prasain, J. K., Dorand, D., & Miller, M. A. (2013). A Heterogeneous Mixture of F-Series Prostaglandins Promotes Sperm Guidance in the Caenorhabditis elegans Reproductive Tract. PLoS Genetics, 9(1), e1003271. https://doi.org/10.1371/journal.pgen.1003271
    Nichols, D. P., Caceres, S., Caverly, L., Fratelli, C., Kim, S. H., Malcolm, K., Poch, K. R., Saavedra, M., Solomon, G., Taylor-Cousar, J., Moskowitz, S., & Nick, J. A. (2013). Effects of azithromycin in Pseudomonas aeruginosa burn wound infection. Journal of Surgical Research, 183(2), 767–776. https://doi.org/10.1016/j.jss.2013.02.003
    Rubio-Guerri, C., Melero, M., Esperón, F., Bellière, E., Arbelo, M., Crespo, J., Sierra, E., García-Párraga, D., & Sánchez-Vizcaíno, J. (2013). Unusual striped dolphin mass mortality episode related to cetacean morbillivirus in the Spanish Mediterranean sea. BMC Veterinary Research, 9(1), 106. https://doi.org/10.1186/1746-6148-9-106
    Lee, J., Brehm, M. A., Greiner, D., Shultz, L. D., & Kornfeld, H. (2013). Engrafted human cells generate adaptive immune responses to Mycobacterium bovis BCG infection in humanized mice. BMC Immunology, 14(1), 53. https://doi.org/10.1186/1471-2172-14-53
    Austin, W. R., Armijo, A. L., Campbell, D. O., Singh, A. S., Hsieh, T., Nathanson, D., Herschman, H. R., Phelps, M. E., Witte, O. N., Czernin, J., & Radu, C. G. (2012). Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress. Journal of Experimental Medicine, 209(12), 2215–2228. https://doi.org/10.1084/jem.20121061
    Hong, N., De-Xing, Q., Deng-Xiang, Z., & Long-Zhong, R. (2012). A Simple Cultural Method for Detection of Mycoplasma bovis. Journal of Animal and Veterinary Advances, 11(10), 1643–1646. https://doi.org/10.3923/javaa.2012.1643.1646
    Kumar, M., Roe, K., Nerurkar, P. V., Namekar, M., Orillo, B., Verma, S., & Nerurkar, V. R. (2012). Impaired Virus Clearance, Compromised Immune Response and Increased Mortality in Type 2 Diabetic Mice Infected with West Nile Virus. PLoS ONE, 7(8), e44682. https://doi.org/10.1371/journal.pone.0044682
    Sanderson, S. D., Thoman, M. L., Kis, K., Virts, E. L., Herrera, E. B., Widmann, S., Sepulveda, H., & Phillips, J. A. (2012). Innate Immune Induction and Influenza Protection Elicited by a Response-Selective Agonist of Human C5a. PLoS ONE, 7(7), e40303. https://doi.org/10.1371/journal.pone.0040303
    Quinton, L. J., Mizgerd, J. P., Hilliard, K. L., Jones, M. R., Kwon, C. Y., & Allen, E. (2012). Leukemia Inhibitory Factor Signaling Is Required for Lung Protection during Pneumonia. The Journal of Immunology, 188(12), 6300–6308. https://doi.org/10.4049/jimmunol.1200256
    El Agha, E., Al Alam, D., Carraro, G., MacKenzie, B., Goth, K., De Langhe, S. P., Voswinckel, R., Hajihosseini, M. K., Rehan, V. K., & Bellusci, S. (2012). Characterization of a Novel Fibroblast Growth Factor 10 (Fgf10) Knock-In Mouse Line to Target Mesenchymal Progenitors during Embryonic Development. PLoS ONE, 7(6), e38452. https://doi.org/10.1371/journal.pone.0038452
    Adhikari, R. P., Karauzum, H., Sarwar, J., Abaandou, L., Mahmoudieh, M., Boroun, A. R., Vu, H., Nguyen, T., Devi, V. S., Shulenin, S., Warfield, K. L., & Aman, M. J. (2012). Novel Structurally Designed Vaccine for S. aureus α-Hemolysin: Protection against Bacteremia and Pneumonia. PLoS ONE, 7(6), e38567. https://doi.org/10.1371/journal.pone.0038567
    Cavasin, M. A., Demos-Davies, K., Horn, T. R., Walker, L. A., Lemon, D. D., Birdsey, N., Weiser-Evans, M. C. M., Harral, J., Irwin, D. C., Anwar, A., Yeager, M. E., Li, M., Watson, P. A., Nemenoff, R. A., Buttrick, P. M., Stenmark, K. R., & McKinsey, T. A. (2012). Selective Class I Histone Deacetylase Inhibition Suppresses Hypoxia-Induced Cardiopulmonary Remodeling Through an Antiproliferative Mechanism. Circulation Research, 110(5), 739–748. https://doi.org/10.1161/CIRCRESAHA.111.258426
    Konsavage, W. M., Zhang, L., Wu, Y., & Shenberger, J. S. (2012). Hyperoxia-induced activation of the integrated stress response in the newborn rat lung. AJP: Lung Cellular and Molecular Physiology, 302(1), L27–L35. https://doi.org/10.1152/ajplung.00174.2011

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